UPLC-Q-TOF/MS^E方法分析养血清脑颗粒的化学成分  

Identification of chemical constituents in Yangxue Qingnao granule by UPLC-Q-TOF/MS^E

作　　者：杨岱琳[1] 佟玲 李晓稳 李东翔 柳文媛[1] 

机构地区：[1]中国药科大学药物分析教研室,江苏南京210009 [2]天士力研究院创新中药关键技术国家重点实验室药物分析研究所,天津300410

出　　处：《药学学报》2016年第5期797-805,共9页Acta Pharmaceutica Sinica

基　　金：国家"重大新药创新"科技重大专项(2013ZX09402202)

摘　　要：为系统阐明复方中药养血清脑颗粒的化学组成,本研究采用超高效液相色谱-四级杆-飞行时间/全信息串联质谱(UPLC-ESI-Q-TOF/MS^E)技术,对养血清脑颗粒的化学成分进行分析。通过Q-TOF-MS^E提供化合物的准确相对分子质量、质谱碎片离子信息,与对照品的保留时间和质谱数据比对,结合参考文献,共鉴定出142种成分,主要包括酚酸类、单萜苷类、醌类、生物碱类和苯酞内酯类5类成分,并对各成分的药材来源进行归属。本研究全面、快速、准确地分析了养血清脑颗粒的化学成分,为养血清脑颗粒的药效物质基础和质量控制等研究奠定了基础。In order to clarify the chemical constituents in Yangxue Qingnao granule,we established a rapid ultra-performance liquid chromatography/orthogonal acceleration time-of-flight mass spectrometry（UPLC-Q-TOF/MS-E） method.According to the high resolution MS spectra data,fragmentation ion information and retention time,142 peaks were identified or tentatively presumed by comparison with reference standards data and literature reports.The herbal sources of these peaks were assigned.The results implied that phenolic acids,alkaloids,anthraquinones,phthalides,monoterpene glycosides were included in the main components of Yangxue Qingnao granule.The method is rapid for systematically elucidation of the constituents of Yangxue Qingnao granule and the results would facilitate the quality control of Yangxue Qingnao granule for safe and efficacious use.

关 键 词：养血清脑颗粒 UPLC-ESI-Q-TOF/MSE 定性分析 

分 类 号：R917[医药卫生—药物分析学]