安徽省281名一般人群外周血淋巴细胞核分裂指数  

Analysis on the nuclear division index of peripheral blood lymphocytes in the 281 general population of Anhui, China

作　　者：滕晶晶[1] 杨同金[2] 叶林[2] 冯学庆[2] 郑玉新[1] 段化伟[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所中国疾病预防控制中心化学污染与健康安全重点实验室,北京100050 [2]安徽省疾病预防控制中心毒理室

出　　处：《中华预防医学杂志》2016年第5期429-433,共5页Chinese Journal of Preventive Medicine

基　　金：国家科技支撑计划（2014BA112B02）;国家自然科学基金（81172642、81573124）

摘　　要：目的研究安徽省一般人群外周血淋巴细胞核分裂指数（NDI）的参考值范围，并分析可能的影响因素。方法2010—2011年在安徽地区招募无职业性遗传毒物接触史及慢性疾病史的一般人群281名。采用调查问卷的方式收集调查对象的年龄、性别、地区、职业暴露史、BMI、吸烟和饮酒状况等信息，采集被调查者外周血，通过胞质分裂阻滞法微核组学计算NDI，分析影响因素，并以NDI为因变量，年龄、性别等因素为自变量采用多重逐步线性回归法进行分析。结果安徽省调查人群的NDI数据为正态分布，NDI为1．71±0．22，最小值为1．10，最大值为2．36。男性人群的NDI值（1．67±0．20）低于女性人群（1．76±0．24），差异有统计学意义（t=-3．65，P〈0．001）；吸烟人群的NDI（1．66±0．18）低于非吸烟人群（1．73±0．24），差异有统计学意义（t=3．06，P=0．002）；饮酒人群的NDI（1．66±0．20）低于非吸烟人群（1．74±0．23），差异有统计学意义（f=3．15，P=0．002）。采用多重逐步线性回归对相关因素进行分析后发现，性别是NDI的独立影响因素，且女性高于男性（β=0．098，sf=0．027，t=3．66，P〈0．001）。结论调查地区的一般人群NDI的参考值范围，可以为同类研究提供参考，可用于评估有害因素对机体的细胞增殖能力的影响。Objective To investigate the reference range and influeing factors of the nuclear division index （NDI） of peripheral blood lymphocyte in Chinese general population in Anhui province. Methods We selected 281 subjects from the general poulation in Anhui province, without occupational exposure to genetic toxicants and no chronic disease history. We used questionnaires to collect occupational history, age, gender, region, body mass index, smoking, and alcohol drinking status etc. NDI was measured by cytokinesis block micronucleus assay in peripheral blood lymphocytes, and the related factors were also analyzed. And NDI was used as the dependent variable, age, gender and other factors as independent variables to conduct stepwise multiple linear regression. Results We found the data of NDI according with normal distribution, and the nuclear division index was 1.71-＋0.22, the minimum value was 1.10 while the maximum was 2.36. The results showed that NDI value of the males （1.67±0.20） were lower than that of the females （1.76±0.24）, the difference was statistically significant （t=-3.65, P〈0.001）; current smokers NDI （1.66±0.18） lower than non-smokers （1.73±0.24） differences were statistically significant （t=3.06, P=0.002）; the NDI of drinking groups （1.66± 0.20） was lower than that of non-drinking population （1.74±0.23）, the differences was statistically significant （t=3.15, P=0.002）; Using multiple stepwise linear regression calibration factors and found that gender was an independent factor of NDI （ β=0.098, sf =0.027, t=3,66, P〈 0.001 ）. Conclusion We set the reference value on the nuclear division index among general population of survey areas in this study, it could provide a reference for similar studies and will provide reference for better evaluation of the effects of hazards on the body.

关 键 词：胞质分裂 淋巴细胞 胞质分裂阻滞 核分裂指数 细胞毒性 横断面研究 

分 类 号：R446.11[医药卫生—诊断学]