γ干扰素(IFN-γ)刺激小鼠肥大细胞释放组织蛋白酶S  

IFN-γ stimulates the release of cathepsin S in mouse mast cells

作　　者：王然[1] 欧阳晴晴 赵进军[1] 黄琴[1] 樊丹冰 杨敏[1] 

机构地区：[1]南方医科大学南方医院风湿免疫科,广东广州510515

出　　处：《细胞与分子免疫学杂志》2016年第5期577-580,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81172875)

摘　　要：目的探讨γ干扰素(IFN-γ)对P815小鼠肥大细胞以及小鼠髓源性肥大细胞(BMMC)表达组织蛋白酶S(CTSS)的影响。方法用(10、25、50)ng/m L IFN-γ分别刺激P815细胞及原代培养的小鼠BMMC,利用实时定量PCR、ELISA分别检测P815细胞及BMMC CTSS的mRNA和蛋白水平。用25 ng/m L IFN-γ处理P815细胞及小鼠BMMC,P815细胞分别处理6、12、18、24、48、72 h,BMMC分别处理12、24、48 h,重复上述检测步骤。结果 IFN-γ可增加P815细胞及BMMC CTSS的mRNA和蛋白水平,并具有一定的时间、剂量依赖关系。结论 IFN-γ可促进小鼠肥大细胞表达CTSS。Objective To investigate the impact of interferonγ（IFN-γ） on cathepsin S （CTSS） expressed by P815 mouse mast cells and mouse bone marrow-derived mast cells （BMMCs）. Methods IFN-γ of 10, 25 and 50 ng/mL were respectively used to stimulate P815 cells and mouse BMMCs. Real-time PCR and ELISA were applied to detect mRNA and protein levels of CTSS in P815 cells and BMMCs. 25 ng/mL IFN-y was used to treat P815 cells for 6, 12, 18, 24, 48 and 72 hours and mouse BMMCs for 12, 24 and 48 hours; the above mentioned detection steps were then repeated. Results IFN-γ induced P815 cells and BMMCs to express CTSS on the mRNA and protein levels in the time- and dose-dependent manners. Conclusion IFN-γ could stimulate mast cells to release CTSS.

关 键 词：P815细胞系 小鼠骨髓源性肥大细胞 IFN-Γ 组织蛋白酶S 

分 类 号：R392.12[医药卫生—免疫学] R446.61[医药卫生—基础医学]