慢性乙型肝炎病毒感染者外周血单个核细胞Toll样受体9水平与CD38、HLA-DR、CD95水平正相关  

TLR9 expression is positively correlated with the levels of CD38,HLA-DR and CD95 on peripheral blood mononuclear cells in chronic HBV infected patients

作　　者：毛学锋 彭丽珊[1,3] 刘显[1,3] 杨洋[1,3] 王启辉[1,4] 王登嵘[1,3] 肖健[1,3] 冷静[1,3] 

机构地区：[1]广西特色实验动物病证模型重点实验室,广西南宁530001 [2]广西玉林市中心血站检验科,广西玉林537000 [3]广西中医药大学医学免疫学与微生物学教研室,广西南宁530001 [4]广西医科大学免疫学教研室,广西南宁530021

出　　处：《细胞与分子免疫学杂志》2016年第5期660-665,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：广西自然科学基金重点项目(2011GXNSFD018031);广西自然科学基金(2012GXNSFAA053125)

摘　　要：目的探讨慢性乙型肝炎病毒(HBV)感染过程中外周血单个核细胞(PBMC)的Toll样受体9(TLR9)表达水平与CD38、HLA-DR、CD95水平的关系。方法募集70例未经抗病毒治疗的慢性HBV感染者,按HBV DNA的含量高低分为高病毒载量组与低病毒载量组,12例健康人作为对照组;采集外周抗凝血,密度梯度法分离PBMC;使用流式细胞术检测PBMC的TLR9、CD38、HLA-DR、CD95的水平。结果慢性HBV感染过程中,高病毒载量组和低病毒载量组PBMC的TLR9、HLA-DR、CD95表达水平显著高于健康对照组;高病毒载量组和低病毒载量组PBMC上TLR9与CD38、HLA-DR、CD95的共表达率显著高于健康对照组;相关性分析结果显示慢性HBV感染者PBMC上,TLR9与CD38、HLA-DR、CD95的表达正相关(r分别为0.345、0.334、0.227)。结论慢性HBV感染过程中,PBMC的TLR9、HLA-DR、CD95表达水平升高,TLR9表达水平与CD38、HLA-DR、CD95水平正相关。Objective To explore the relationship between the expression of TLR9 and the levels of CD38, HLA-DR and CD05 on peripheral blood mononuclear cells （PBMCs） of chronic hepatitis B virus （HBV） infected patients. Methods 70 chronic HBV infected patients and 12 healthy donors were enrolled in this study, and density gradient centrifugation was used to isolate PBMCs from peripheral blood with EDTA for anticoagulation. Flow cytometry was used to detect the levels of TLRg, CD38, HLA-DR and CD05 on PBMCs. Results Compared to the healthy donors, chronic HBV infected patients with low viral load or high viral load had significantly higher levels of TLRg, HLA-DR and CD95 on PMBCs. Furthermore, the co-expression rates of TLR9 and CD38, HLA-DR, CD05 on PBMCs were obviously higher than those of the healthy donors. Correlation analysis showed that the expression of TLR9 was positively correlated with CD38 （r=0. 345）, HLA-DR （r =0. 334）, CD05 （r=0.227） on PBMCs in the patients with chronic HBV infection. Conclusion The expression of TLR9 increased and was positively associated with CD38, HLA-DR and CD05 on PBMCs during chronic HBV infection.

关 键 词：乙型肝炎病毒 Toll样受体9(TLR9) CD38 HLA-DR CD95 

分 类 号：R512.62[医药卫生—内科学] R392.12[医药卫生—临床医学]