晚期非小细胞肺癌患者外周血固有样淋巴细胞亚群比例变化和临床意义  

Changes and clinical significance of innate-like lymphocyte subsets in peripheral blood of patients with advanced non-small cell lung cancer

作　　者：魏婷婷[1] 韩啸[2] 夏晓影[1] 马芳[3] 姚春艳[1] 

机构地区：[1]蚌埠医学院医学检验系免疫学教研室,安徽省感染与免疫重点实验室 [2]蚌埠医学院医学第一附属医院肿瘤内科 [3]蚌埠医学院医学检验系临床检验诊断学教研室,安徽蚌埠233000

出　　处：《细胞与分子免疫学杂志》2016年第5期671-675,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：安徽省教育厅大学生创新创业训练项目(AH201410367021);蚌埠医学院科研项目(Byky1327)

摘　　要：目的检测固有样淋巴细胞、αβT细胞、B2细胞在非小细胞肺癌(NSCLC)患者和正常人体内的分布情况及相关性。方法收集16例正常对照和5例NSCLC初诊患者和15例病情稳定患者外周血,采用流式细胞术检测外周血淋巴细胞中,恒定型自然杀伤T(i NKT)细胞、γδT细胞、B1细胞及αβT细胞、B2细胞的比例。结果病情稳定组的i NKT细胞比例显著低于对照组和初诊患者组,而αβT细胞比例显著高于对照人群;初诊患者组的γδT细胞显著高于病情稳定组;初诊患者组B1细胞比例明显低于对照组。病情稳定组i NKT细胞和γδT细胞呈正相关;病情稳定组和对照组B1和B2细胞呈正相关。结论NSCLC患者外周血固有样淋巴细胞亚群失衡。Objective To investigate the percentages and correlations of innate-like lymphocyte subsets, αβT cells and 132 cells in peripheral blood of patients with non-small cell lung cancer （NSCLC） and normal individuals. Methods A total of 16 healthy controls, 5 NSCLC first-visit patients and 15 NSCLC stable-state patients were included to take the peripheral blood samples. Flow cytometry was performed to detect the percentages of invariant natural killer T （ iNKT）, γδT, B1, αβT and B2 cell subsets in peripheral blood lymphocytes. Resets The percentage of iNKT cells in the stable-state patients was significantly lower than that of the healthy people and first-visit patients. In addition, the percentage of αβT cells in the stable-state patients was significantly higher than that of the healthy people. The fist-visit patients had a markedly higher percentage of γδT cells than the stable-state patients, but a significantly lower percentage of B1 cells than the healthy people. There was an obviously positive correlation between iNKT cells and yST cells in the stable-state patients. We also found a significantly positive correlation between BI cells and B2 cells in both stable-state patients and healthy people. Conclusion The percentages of innate-like lymphocyte subsets in patients with NSCLC are in disequilibrium.

关 键 词：非小细胞肺癌 固有样淋巴细胞 流式细胞术 

分 类 号：R392.9[医药卫生—免疫学] R734.2[医药卫生—基础医学]