骨髓间充质干细胞旁分泌效应对胰腺β细胞增殖、凋亡及分泌功能的影响  

Influence of paracrine effects of bone mesenchymal stem cells on proliferation,apoptosis and secretion function of pancreatic β cell line

作　　者：许聿新 井庆平 赵翠红 

机构地区：[1]淄博市第一医院,山东淄博255200

出　　处：《山东医药》2016年第14期1-3,共3页Shandong Medical Journal

基　　金：山东省淄博市科学技术发展计划项目(2012GG01259)

摘　　要：目的观察骨髓间充质干细胞(BMSCs)旁分泌效应对胰腺β细胞系INS-1细胞增殖、凋亡及胰岛素分泌的影响。方法培养BMSCs,以胰腺提取物(RPE)模拟胰腺微环境培养BMSCs后,获取BMSCs的条件培养液(BMSCs-CM)。将细胞分为A组、B组、C组,分别以BMSCs-CM、不含RPE的条件培养液、等量RPMI 1640培养液培养。采用MTT法观察各组细胞增殖情况,计算细胞增殖率。采用AnnexinⅤ/PI法观察各组细胞凋亡情况,计算细胞凋亡率。应用放射免疫法(RIA)测定各组胰岛素水平。结果 A组细胞增殖率高于B组及C组(P均<0.05)。A组细胞凋亡率低于B组及C组(P均<0.05)。在葡萄糖溶液终浓度为5.6及20.0 mmol/L时,A组胰岛素水平均高于B组及C组(P均<0.05)。结论 BMSCs-CM培养INS-1细胞后可促进其增殖、抑制其凋亡并促进其分泌胰岛素,BMSCs可能通过旁分泌效应发挥以上作用。Objective To investigate the influence of paracrine effects of bone mesenchymal stem cells （BMSCs） on the proliferation, apoptosis and insulin secretion of pancreatic β cell line INS-1 cells. Methods The BMSCs were cultured and BMSCs conditioned media （BMSCs-CM） were obtained by culturing BMSCs with rat pancreatic extracts （RPE） which simulated the pancreatic The INS-1 cells were divided into groups A, B and C, which were cultured with BMSCs-CM, conditioned media without RPE and RPMI 1640 culture solution, respectively. The influence of BMSCs-CM on INS-1 cell proliferation was analyzed by MTT. The cell proliferation rate was calculated. The effects of BMSCs-CM on the INS-1 cell apoptosis were measured by AnnexinV/PI double staining. The rate of apoptosis was counted. The insulin concentration of INS-1 cells was analyzed by radioimmunoassay （RIA）. Results The cell proliferation rate in the group A was higher than that in group B and C （P 〈 0. 05）. The apoptosis rate in the group A was lower than that in group B and C （P 〈 0. 05）. The insulin concentration in the group A was higher than that in group B and C at the concentration of 5.6 mmol/L and 20.0 mmol/L （all P 〈 0.05）. Conclusions The incubation of BMSCs-CM can stimulate the INS-1 cell proliferation, inhibit the apoptosis, and promote the insulin secretion. The above actions of BMSCs may be achieved by paracrine effects.

关 键 词：干细胞 骨髓 胰腺p细胞 细胞因子 凋亡 增殖 

分 类 号：R587.1[医药卫生—内分泌]