聚丙烯酸功能化量子点荧光探针测定ct-DNA  

Poly(acrylic acid)-functionalized quantum dots fluorescent probe for ct-DNA detection

作　　者：张艺[1,2] 高红艳[2] 黄珏欣[2] 

机构地区：[1]伊犁师范学院新疆凝聚态相变与微结构实验室,新疆伊宁835000 [2]伊犁师范学院化学与环境科学学院,新疆伊宁835000

出　　处：《分子科学学报》2016年第2期167-171,共5页Journal of Molecular Science

基　　金：新疆维吾尔自治区高等学校科研计划资助项目(XJEDU2013S40)

摘　　要：以聚丙烯酸功能化的Cd S：Mn量子点为荧光探针,基于核酸对量子点强度的荧光猝灭,实现了对DNA的定量测定.在p H=6.6条件下,功能化量子点的荧光强度和小牛胸腺DNA（ct-DNA）的浓度（0.20-1.50 mg/L）成线性关系,线性方程为ΔF=173.99-62.02c,检出限为0.037 mg/L,相关系数R=0.999 4,RSD为1.28%（n=5）.此方法快速、选择性好.Based on the quenching effect of the nucleic acid on the Quantum Dots（QDs） fluorescence, the Poly（acrylic acid）-Functionalized Cd S：Mn QDs as fluorescence probe to quantitatively detect the DNA. The optimal p H is 6.6 and the fluorescence intensity of the functionalized QDs is linearly proportional to the concentration of ct-DNA from 0.20 to 1.50 mg/L. the linear equation is ΔF=173.99-62.02 c, the limit of detection is 0.037 mg/L, and the correlation coefficient is 0.999 4, the relative standard deviation is 1.28%（n=5）. It shows that the method is rapid and sensitive.

关 键 词：小牛胸腺DNA(ct-DNA) 量子点 荧光猝灭 

分 类 号：O652[理学—分析化学]