按部选穴针刺治疗对糖尿病胃轻瘫大鼠延髓内c-fos和神经胶质酸性蛋白表达的影响  

Effects of acupuncture at different acupoints on c-fos and glial fibrillary acidic protein expression in the medulla oblongata of rats with diabetic gastroparesis

作　　者：刘丽[1] 陈小丽[1] 文琪琦[1] 李萍[1] 魏歆然[1] 王艳[1] 岳增辉[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南省长沙市410007

出　　处：《世界华人消化杂志》2016年第11期1701-1707,共7页World Chinese Journal of Digestology

基　　金：国家重点基础研究计划基金资助项目;No.2014CB543102~~

摘　　要：目的:观察按部选穴针刺治疗对糖尿病胃轻瘫(diabetic gastroparesis,DGP)模型大鼠延髓内c-fos和神经胶质酸性蛋白(glial fibrillary acidic protein,GFAP)表达的影响,探讨按部选穴是腧穴配伍效应的影响因素.方法:60只SD大鼠随机分为空白对照组、模型组、针刺足三里+中脘组、针刺足三里+内关组、针刺足三里+非经非穴组,每组12只.采用单次腹腔注射2%链脲佐菌素配合普通饲料饮食建立DGP模型大鼠,造模成功后,连续治疗4 wk.治疗结束后,用黑墨水灌胃测小肠推进率,用免疫组织化学法检测大鼠延髓内c-fos和GFAP的灰度值.结果:与空白对照组比较,模型组小肠推进率显著降低,c-fos和GFAP的灰度值降低,有统计学意义(P<0.05).与模型组比较,针刺足三里+中脘组、针刺足三里+内关组、针刺足三里+非经非穴组小肠推进率显著升高,针刺足三里+中脘组c-fos和GFAP的灰度值显著升高,有统计学意义(P<0.05).与针刺足三里+中脘组比较,针刺足三里+内关组、针刺足三里+非经非穴组c-fos和GFAP的灰度值显著降低,有统计学意义(P<0.05).结论:针刺可改善DGP模型大鼠胃排空迟缓症状,调节延髓内c-fos和GFAP的表达,配伍局部腧穴显著优于配伍远端腧穴及非经非穴点,表明不同按部选穴是影响腧穴配伍效应的关键因素.AIM： To observe the effects of acupuncture at different acupoints on the expression of c-fos and glial fibrillary acidic protein（GFAP） in the medulla oblongata of rats with diabetic gastroparesis（DGP）, and to discuss whether the ministry of acupoints is the factor influencing the effect of acupoints compatibility.METHODS： Sixty SD rats were randomly divided into a normal group, a model group,a Zusanli ＋ Zhongwan group, a Zusanli ＋Neiguan group, and a Zusanli ＋ non acupoint group, with 12 rats in each group. DGP was induced by intraperitoneal injection of streptozotocin（STZ）（60 mg/kg, 2%） and treated for 4 wk continuously. After treatment, small intestinal transit rate was measured with black ink, and the expression of c-fos and GFAP in the medulla oblongata was detected by immunohistochemistry.RESULTS： In comparison with the normal control group, small intestinal transit rate of the model group was significantly decreased,and the gray values of c-fos and GFAP were decreased（P〈 0.05）. In comparison with the model group, small intestinal transit rates of the Zusanli ＋ Zhongwan group, Zusanli ＋ Neiguangroup, and Zusanli ＋ non acupoint group were significantly increased, and the gray values of c-fos and GFAP were significantly increased（P 〈0.05）. In comparison with the Zusanli ＋Zhongwan group, the gray values of c-fos and GFAP in the Zusanli ＋ Neiguan group and Zusanli ＋ non acupoint group were significantly decreased（P 〈0.05）.CONCLUSION： Acupuncture can improve the symptoms of delayed gastric emptying in DGP rats, and regulate the expression of c-fos and GFAP in the medulla. The compatibility of local acupoints is significantly better than the compatibility of distal acupoints and nonpoints. Acupoint selection is the key factor affecting acupoint compatibility.

关 键 词：腧穴配伍 按部选穴 糖尿病胃轻瘫 C-FOS 神经胶质酸性蛋白 

分 类 号：R245[医药卫生—针灸推拿学]