拟南芥低叶绿素荧光LCF3基因的克隆与功能分析  

Map-based Cloning and Functional Analysis of Low Chlorophyll Fluorescence Gene LCF3 in Arabidopsis thaliana

作　　者：刘凌云[1] 刘浩[1] 赵晶[1] 王艳霞[1] 王棚涛[1] 

机构地区：[1]河南大学生命科学学院/棉花生物学国家重点实验室/植物逆境生物学重点实验室,河南开封475004

出　　处：《作物学报》2016年第5期690-695,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31170253);河南省基础与前沿技术研究计划项目(142300413206)资助~~

摘　　要：叶绿素荧光动力学技术在测定叶片光合作用中光系统对光能的吸收、传递、耗散、分配等方面具有独特的作用。本研究借助叶绿素荧光成像仪,从拟南芥EMS诱变突变体库中筛选到一株低叶绿素荧光突变体lcf3-1(lower chlorophyll fluorescence 3-1)。遗传分析表明lcf3-1突变体为单基因隐性突变。突变基因图位克隆结果显示LCF3是Psb W的等位基因。另外,LCF3基因的T-DNA插入突变体及功能回补转基因植物的叶绿素荧光分析结果均证明LCF3基因突变导致拟南芥叶绿素荧光F_v/F_m值降低。进一步实验结果显示,LCF3蛋白定位于叶绿体,且LCF3基因在植株中普遍表达。PsbW蛋白可能细微调整PSII-LHCII超复合体的组装及稳定。Chlorophyll fluorescence has exclusive role for determining the kinetics of light energy absorption, transmission, dissipation and distribution in leaf photosynthesis. In this study, a mutant was screened by chlorophyll fluorescence equipment from EMS mutagenesis of wild-type Arabidopsis Col-0, which showed lower Fv/Fm than wild-type and was named lcf3-1（lower chlorophyll fluorescence 3-1）. Genetic analysis of lcf3-1 mutant suggested that the mutation was controlled by single recessive gene. LCF3 gene, which encodes Psb W protein, was isolated by map-based cloning. The T-DNA insertion mutant lcf3-2 showed low chlorophyll fluorescence phenotype as lcf3-1, and this phenotype could be complemented by LCF3 gene of wild-type. These experiments implied that the phenotype of low-chlorophyll-fluorescence resulted from LCF3 gene mutation. Furthermore, our results indicated that LCF3 protein is located in chloroplast and LCF3 gene is expressed in various tissues. PsbW protein is important for the contact and stability between several PSII-LHCII supercomplexes.

关 键 词：拟南芥 叶绿素荧光 光合作用 图位克隆 

分 类 号：Q943.2[生物学—植物学]