三种不同材料人工晶状体的囊膜生物相容性比较  

Comparison of the capsule biocompatibility of three types of intraocular lenses in vitro

作　　者：黄琪[1] 赵健[2] 党亚龙[2] 郑伯文 胡志鹏 王桂琴[1] 

机构地区：[1]南方医科大学第三临床医学院眼科,广州510515 [2]香港大学眼科医学院脑干视网膜实验室,中国香港999077 [3]香港激光眼科中心,中国香港999077

出　　处：《中华实验眼科杂志》2016年第5期402-407,共6页Chinese Journal Of Experimental Ophthalmology

摘　　要：背景研究表明，后囊膜混浊（PCO）的发生与人工晶状体（IOLs）的材料有关，然而不同材料的IOLs对PCO发生率的影响存在争议。目的研究不同材料IOLs表面人晶状体上皮细胞（LECs）的黏附、增生、上皮-间充质转化（EMT）和相关细胞因子分泌情况，探讨不同材料IOLs的囊膜生物相容性。方法将人LECs系HLE—B3细胞分别接种于疏水性丙烯酸酯（AcrysofSA60AT）IOLs、硅凝胶（Crystalens）IOLs、聚甲基丙烯酸甲酯（PMMA）IOLs的表面培养6h和24h，观察不同IOLs表面黏附的细胞数目及其形态；采用细胞计数试剂盒-8（CCK-8）检测IOLs表面细胞的增生反应；采用免疫荧光技术检测不同IOLs表面人LECs间质细胞标志物a-平滑肌肌动蛋白（0【-SMA）的表达，计算IOLs表面人LECs的EMT转化率；采用ELISA法检测不同材料IOLs表面细胞培养液中转化生长因子-β2（TGF—β2）、白细胞介素-6（IL-6）、基质金属蛋白酶-2（MMP-2）及MMP-9的质量浓度。结果细胞接种于不同材料IOLs并培养6h，AcrysofIOLs和CrystalensIOLs表面黏附的细胞以多边形和椭圆形为主，而PMMAIOLs表面黏附的细胞形态以纺锤形为主；培养后24h，IOLs表面细胞全部伸展，AcrysofIOLs和CrystalensIOLs表面黏附的细胞为多边形，长梭形细胞增多，PMMAIOLs表面细胞呈典型的纤维纺锤样细胞，部分细胞聚集成团。培养后24hAcrysofIOLs、CrystalensIOLs和PMMAIOLs表面的增生相对细胞数A值分别为0．2384±0．0071、0．1781±0．0066和0．1589±0．0069，总体比较差异均有统计学意义（F=475．947，P=0．000），其中AcrysofIOLs表面的细胞数明显多于CrystalensIOLs和PMMAIOLs，差异均有统计学意义（均P〈0．001）。AcrysofIOLs、CrystalensIOLs和PMMAIOLs表面人LECs的EMT转化率分别为（9．99±3．80）％、（17．33±5．71）％和（84．16±10．48）％，组间总体比较差异有统计学意义（F=127．411，P=0．000），其Background Researchers showed that posterior capsule opacification （PCO） may be associated with the materials of intraocular lenses （IOLs）. However, there have long been controversies about the influence of IOLs materials on PCO pathogenesis. Objective This study was to compare the capsule biocompatibility of different materials of IOLs by observing the biological behavior of human lens epithelial cells （LECs） on the surfaceof IOLs,including adhesion,proliferation, epithelial mesenchymal transition （EMT） and the secretion of transforming growth factor-β2 （ TGF-β2 ）, interleukin-6 （ IL-6 ）, matrix metalloproteinase-2 （ MMP-2 ） and MMP-9 in vitro. Methods Human lens epithelial cell line （HLE-B3） was cultured on the surface of hydrophobic acrylic （Acrysof SA60AT） IOLs,silicone （Crystalens HD） IOLs and polymethyl methacrylate （PMMA） IOLs for 6 hours and 24 hours,respectively. The number and morphology of HLE-B3 cells on the surface of IOLs were observed under the optical microscope. The proliferation states of the ceils on the IOL surface were detected with cell counting kit-8 （CCK-8）. The expression of a-smooth muscle actin （a-SMA） , a mesenchymal cell marker,in the cells was detected by immunofluorescence technology, and the EMT rates of HLE-B3 cells were calculated. The contents of TGF-β2 , IL-6, MMP-2 and MMP-9 in the medium of IOL surface were measured by ELISA assay. The examination results were compared among different IOLs. Results Six hours after cultured, attached cells showed polygon and round in shape on the surface of Acrysof IOLs and Crystalens IOLs, while those on the PMMA IOLs showed the fusiform. Twenty-four hours after cultured, the ceils extended obviously. On the surface of Acrysof IOLs and Crystalens IOLs, the adherent cells showed less cobble-stone like and more spindle shape;while those on the PMMA IOLs showed the typical fiber and some cells clustered the pearl roils. The number of the ceils on the Acrysof IOLs, Crystalens IOLs and PMMA IO

关 键 词：生物相容性材料 囊膜混浊/病因 人工晶状体/不良反应 晶状体后囊膜/病理 晶状体 上皮细胞 人 

分 类 号：R318.1[医药卫生—生物医学工程] R779.6[医药卫生—基础医学]