穴位埋线调节变应性鼻炎模型大鼠血清Th细胞因子平衡状态的实验研究  

The experiment research of catgut implantation at acupoints adjust the balance of The cytokines on the rat model with allergic rhinitis

作　　者：陈晴[1] 刘洋[1] 张勤修[2] 李昕蓉[1] 

机构地区：[1]成都中医药大学,四川成都610072 [2]成都中医药大学附属医院耳鼻咽喉科

出　　处：《中国中西医结合耳鼻咽喉科杂志》2016年第1期1-4,共4页Chinese Journal of Otorhinolaryngology in Integrative Medicine

基　　金：国家自然科学基金面上项目(No.81273985);高等学校博士点学科专项科研基金项目(No.20125132110011);四川省国际科技合作与交流研究计划项目(No.2013HH0021)

摘　　要：目的探讨穴位埋线对变应性鼻炎实验动物外周血清的sIgE、IL-4、IL-10、INF-γ水平的影响及其作用机制。方法用卵清蛋白(OVA)建立AR大鼠模型,实验组大鼠穴位埋线后2周,处死动物采血,取血清送检。用酶联免疫吸附试验法(ELISA法)对各组大鼠外周血清sIgE、IL-4、IL-10、INF-γ变化进行观察。结果 1模型组大鼠外周血清sIgE、IL-4、IL-10水平明显升高,与空白组相比有明显差异(P<0.05);INF-γ水平升高,与空白组相比没有明显差异(P>0.05)。2实验组大鼠外周血清sIgE、IL-4、IL-10水平显著下降,与模型组相比有统计学意义(P<0.05),与空白组相比没有显著差异(P>0.05);INF-γ水平显著升高,与空白组、模型组相比有显著差异(P>0.05)。结论穴位埋线可以通过调节变应性鼻炎大鼠血清sIgE、IL-4、IL-10和INF-γ水平,调节Th1/Th2水平,改善变应性鼻炎症状。Objective To investigate the effect of catgut implantation at acupoints on sIgE IL-4, IL-10, sIgE and INF-γ, as well as its mechanism in a rat model with allergic rhinitis. Method Ovalbumin（OVA）- sensitized rats were randomly assigned into three groups. After two weeks of catgut implantation at acupoints in the experimental group, serum samples were collected. The levels of IL-4, IL-10, sIgE and INF-γ were measured by enzyme-linked immunosorbent assay（ELISA） method. Result ①Serum IL-4, IL-10 and sIgE were significantly increased in the model group in comparison with the blank group（P〈0.05）; INF-γ also increased in the model group, but without significant difference（P〉0.05）. ②Serum IL-4, IL-10 and s Ig E decreased significantly in experimental group in comparison with the model group（P〈0.05）, but there were no significant difference compared with the blank group（P〉0.05）;INF-γ increased significantly, in comparison with the blank model group（P〉0.05）. Conclusion The catgut implantation at acupoints might improve the symptoms of allergic rhinitis by regulating the level of serum IL-4, IL-10, sIgE and INF-γ.

关 键 词：穴位埋线 变应性鼻炎 大鼠模型 细胞因子 

分 类 号：R245[医药卫生—针灸推拿学]