四种常用实验麻醉药物对大鼠心血管系统的影响  

Impact of four anesthetic drugs commonly used in animal experiments on the cardiovascular system in rats

作　　者：孙安会[1] 谷捷[1] 吴涛[1] 袁肇凯[1] 蔡雄[1] 胡志希[1] 简伟雄[1] 李鑫[1] 

机构地区：[1]湖南中医药大学,长沙410007

出　　处：《中国实验动物学报》2016年第2期120-126,共7页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金项目(编号:81273670);教育部博士点基金项目(编号:20124323130001);湖南省研究生创新基金重点项目(编号:CX2014A014)

摘　　要：目的探讨不同动物实验麻醉药物对大鼠心血管系统的影响。方法采用Bio Pac MP150动态记录四种麻醉药物麻醉后大鼠的心电图、血压,采集麻醉后不同时间点大鼠的血糖以及实验终点肝肾功能、心肌酶、电解质。结果水合氯醛可引起严重的室性心律失常,异氟烷对心率有抑制作用,戊巴比妥钠可引起大鼠心电图P波增高,乌拉坦可引起大鼠心电图J点抬高。乌拉坦、戊巴比妥钠均可引起SBP升高,水合氯醛可引CK升高,异氟烷可降低CK、CKMB,戊巴比妥钠、异氟烷有降低ALT、AST的作用,水合氯醛、戊巴比妥钠、异氟烷均可降低肌酐,四种麻醉药物均有降低血钠、血钾的作用。结论水合氯醛对心血管系统的影响明显,不适合用于心血管病的动物实验研究,戊巴比妥钠、乌拉坦、异氟烷可适当选择作为心血管病的动物实验研究。Objective To explore the impact of four different anesthetic drugs commonly used in animal experiments on cardiovascular system in rats. Methods Electrocardiogram（ ECG） and blood pressure were dynamically recorded by a Bio Pac MP150 system after anesthesia. In addition,the blood glucose at different time points and hepatic function,kidney function,cardiac enzymes and electrolytes at the end of the test were collected. Rusults Chloral hydrate caused severe ventricular arrhythmia. Isoflurane had inhibitory effect on the heart rate. Pentobarbital sodium induced a increase of ECG P wave. Urethane caused J point elevation of ECG. Blood pressure in the urethane- and pentobarbital sodium-treated groups were increased. Chloral hydrate caused CK to be raised,while isoflurane showed the opposite effect on CK and CKMB. Alanine aminotransferase and aspartate aminotransferase in the pentobarbitol sodium and isoflurane groups were decreased. Creatinine in the chloral hydrate,pentobarbital sodium and isoflurane groups were lower,and the serum sodium and potassium were decreased in the four groups. Conclusions Chloral hydrate has obvious effect on the cardiovascular system,and is not suitable for animal studies on cardiovascular diseases. Pentobarbital sodium,urethane,isoflurane can be chosen for animal studies on cardiovascular diseases.

关 键 词：动物实验 麻醉药物 大鼠 心血管系统 

分 类 号：Q95-33[生物学—动物学]