苯胺致小鼠肝细胞和淋巴细胞DNA损伤及其修复效应  

DNA damages in mouse hepatocytes and lymphocytes induced by aniline and their repair dynamics

作　　者：边高鹏[1,2] 刘瑞祥[1,2] 史宝忠[1,2] 焦海华[1] 

机构地区：[1]长治学院生物科学与技术系,山西长治046011 [2]太行山生态与环境研究所,山西长治046011

出　　处：《中国实验动物学报》2016年第2期139-144,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：中国科学院环境生物技术重点实验室开放研究基金(编号:EBT2013A001);山西省高等学校大学生创新创业训练重点项目(编号:2015426)

摘　　要：目的研究苯胺的遗传毒性及其修复动力学效应。方法应用单细胞凝胶电泳(SCGE)技术,检测100 mg/kg苯胺单次灌胃3、8、16、24、32 h后,对KM小鼠肝细胞和淋巴细胞DNA损伤及时效关系。结果 SCGE实验结果显示肝细胞从8 h开始尾长和尾矩逐渐增大,至16 h DNA损伤程度达到最大,相比对照组差异有显著性(P<0.01),随着时间的延长,DNA损伤程度逐渐减轻,在32 h DNA损伤已恢复正常,与对照组相比差异无显著性(P>0.05);而淋巴细胞则在16 h开始尾长和尾矩逐渐增大,24 h时达到最大,32 h时DNA损伤逐渐恢复。结论苯胺对肝细胞和淋巴细胞具有潜在的遗传毒性;2个DNA损伤指标的变化存在明显的时间效应关系,说明这两种细胞具有有效DNA修复机制。Objective To investigate the genotoxicity of aniline and repair dynamics in hepatocytes and lymphcytes. Methods Aniline was administered intragastrically to SPF Kunming mice（ five mice in each group） in a single dose of 100 mg / kg body weight. The hepatocytes and peripheral blood lymphocytes were obtained at 3,8,16,24,and 32 hours after aniline administration,respectively. The control mice received tap water only. The DNA damages were detected by single cell gel electrophoresis assay（ SCGE） and the time-effect relationship was analyzed. Results The results of SCGE experiment showed that both the tail lenth and tail moment of the hepatocyte DNA were increased gradually from 8 h,and reached the maximum at 16 h（ P〈0. 01） after aniline administration. As time went on,DNA damage was recovered gradually,and the two DNA damage indexes were completely returned to control levels at 32 h after aniline administration（ P〉0. 05）. The two DNA damage indexes of peripheral blood lymphocytes started to increase at 16 h,reached the maximum at 24 h（ P〈0. 01）,and began to recover at 32 h after aniline administration. Conclusions Our findings suggeste that aniline may be a potential genotoxicant to hepatocytes and peripheral blood lymphocytes. There is a clear time-response relationship in terms of the two DNA damage indexes,indicating that hepatocytes and lymphocytes in mice possess an efficient DNA repair mechanism against aniline toxicity.

关 键 词：苯胺 单细胞凝胶电泳 DNA修复 

分 类 号：Q95-33[生物学—动物学]