Fn14诱导人皮肤成纤维细胞分泌细胞外基质  

The induce effect of Fn14 on the secretion of extracellular matrix by human skin fibroblast

作　　者：麦丽萍[1,2] 陈少贤[2] 肖定璋[2] 陈景[2] 杨敏[2] 余细勇[2] 刘培庆[1] 

机构地区：[1]中山大学药学院,广东广州510006 [2]广东省人民医院,广东省医学科学院医学研究部,广东广州510080

出　　处：《热带医学杂志》2016年第4期415-417,447,共4页Journal of Tropical Medicine

基　　金：广东省科技计划项目(2014A020212226,2014A020212674,2015B020225006);广州市科技计划项目(201510010210);广东省医学科研基金(A2014028)

摘　　要：目的研究成纤维细胞生长因子诱导分子-14(Fn14)对人皮肤成纤维细胞(HSF)分泌细胞外基质的影响,探讨皮肤纤维化新的干预环节。方法分离HSF,构建Fn14表达载体,将其转染HSF,用Western blot方法检测Fn14、平滑肌肌动蛋白α(α-SMA)和Ⅰ型胶原(ColⅠ)蛋白表达水平;Real-time PCR(RT-qPCR)检测Ⅰ型和Ⅲ型胶原(ColⅢ)m RNA表达水平。结果构建的Fn14表达载体可成功转染HSF细胞,并使HSF细胞α-SMA和Ⅰ、Ⅲ型胶原表达水平升高。结论 Fn14能促进HSF胶原的表达,提示其在促进皮肤纤维化的病理过程中起着重要的作用。Objective To study the effect of fibroblast growth factor-inducible molecule 14（Fn14） on the secretion of extracellular matrix by human skin fibroblast（HSF）, and explore new intervention node on cell fibrosis. Methods HSF were isolated. Fn14 expression vector was constructed and transfected into HSF cells. The protein expression levels of Fn14, α-SMA and collagen Ⅰ were detected by western blot assay. The m RNA levels of collagen Ⅰ and collagen Ⅲ were detected by RT-q PCR assay. Results The Fn14 expression vector was effectively transfected into HSF and can increased the expression of α-SMA, collagen Ⅰ and collagen Ⅲ. Conclusion Fn14 can promote the expression of collagen in HSF suggesting that it plays an important role in promoting the pathological process of skin fibrosis.

关 键 词：Fn14 成纤维细胞 细胞外基质 纤维化 

分 类 号：Q512.6[生物学—生物化学]