胰岛因子1在牙胚不同发育时期对上颌牙髓神经投射表达的影响  

Isl1 influence in different periods of the maxillary nerve projection

作　　者：王瑶[1] 姬晓炜[2] 贺瑞[3] 王进涛[3] 张琼[3] 张远[3] 张蕾[3] 张鹏涛[3] 钟良军[3] 

机构地区：[1]新疆医科大学第一附属医院牙周黏膜科,新疆乌鲁木齐830000 [2]新疆医科大学第一附属医院修复科,新疆乌鲁木齐830000 [3]杭州师范大学附属医院,浙江杭州310000

出　　处：《临床口腔医学杂志》2016年第4期195-198,共4页Journal of Clinical Stomatology

基　　金：国家自然科学基金(81570989)

摘　　要：目的:探讨胰岛因子1(Islet1,Isl-1)在上颌牙牙髓神经生长发育的3个不同时期中投射表达的影响。方法:用Wnt1^(cre/+)雄鼠与Isl1^(f/f)雌鼠配出Wnt1^(cre/+);Isl1^(-/-)基因敲除的小鼠,做基因鉴定(PCR)。取发育不同时期第13.5d、14.5 d、15.5 d小鼠胚胎头部(实验组),同窝非基因敲除小鼠为对照,冰冻切片。用免疫荧光方法标记牙髓神经,比较第13.5 d、14.5 d、15.5 d小鼠上颌牙胚在基因敲除小鼠中与同窝对照小鼠中神经分布情况。结果:PCR结果鉴定为基因敲除小鼠以及同窝对照小鼠;分别在第13.5 d、14.5 d、15.5 d观察基因敲除小鼠与同窝对照小鼠牙髓神经,且各个时期的实验组中神经分布明显减少,对照组中神经分布较多。结论:在牙髓神经的生长发育过程中,Isl-1这一转录因子对牙髓神经的投射表达产生影响,可能是Isl-1调控牙胚周围的转录因子,并影响牙髓神经的投射。Objective：To investigate the effect of islet factor 1（Islet1,Isl-1）on the expression of the three different stages of nerve growth in the dental pulp of maxillary teeth. Method：Bring Wnt1^（cre/＋）male with bring Isl1^（f/f）female together to get the next generation bring Wnt1^（cre/＋）;Isl1^（-/-）,use the PCR to appraisal the next generation genotype. Get the mouse brain respectively at 13.5 days 、14.5 days and 15.5 days post conception. The control is the same group not Isl1 null mouse brain.Frozen section this tissue. Comparison of nerve distribution of the 13.5 days,14.5 days,15.5 days of mice by using the method of immunofluorescence staining in the mice with gene knockout mice and the same group the mice control. Result：Identification of the PCR results for gene knock in mice and littermate control mice,respectively in section 13.5 days,day14.5 and 15.5 days to observe the gene knock in addition to mice and littermate control mice dental pulp nerve,and each period of the experimental group in the nerve distribution significantly reduced,the control group nerve distribution more.Conclusion：During the growth and development of dental pulp,the transcription factor of Isl-1 may affect the expression of the nerve of the pulp,which may be the transcription factor of Isl-1,and affect the projection of the pulp nerve.

关 键 词：牙神经 Isl-1 生长发育 

分 类 号：R322.4[医药卫生—人体解剖和组织胚胎学]