风湿宁胶囊的质量控制研究  

Study of the quality control for Fengshining capsules

作　　者：陈飞[1] 段秀俊[1] 金龙[1] 薛慧清[1] 史静超[1] 周然[1] 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《山西中医学院学报》2016年第2期35-38,共4页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家科技重大专项重大新药创制项目(2010ZX09102-210);山西省科技产业化环境建设项目(2012061014-3)

摘　　要：目的:建立风湿宁胶囊的鉴别方法及含量测定方法。方法:鉴别采用薄层色谱法,含量测定采用高效液相色谱法。结果:独活、青风藤、血竭与肉桂的薄层鉴别图谱中,斑点清晰,阴性无干扰;血竭及青风藤的HPLC测定方法,血竭素线性范围0.116μg^0.581μg,Y=3×10~6X-13 854(r=0.999 6),平均回收率为100.86%,RSD为2.14%(n=6);青藤碱线性范围0.2μg^1.6μg,Y=18.13X-0.005 5(r=0.999 1),平均回收率为100.96%,RSD为2.34%(n=6)。结论:鉴别及含量测定方法专属性强,重复性好,准确度高,可用于风湿宁胶囊的质量控制。Objective：To establish the methods for identification and content determination of Fengshining capsules.Methods：TLC was used for identification,HPLC was used for determination. Results：Spots of Radix Angelicae,Caulis Sinomenii seu Diploclisiae,Resina Draconis and Casssia Barktree were clear,and the blank test didn′ t show interference.Linear range of dracorhodin was 0.116 μg ~0.581 μg,the regression equation was Y =3 ×10~6X-13 854（r =0.999 6）,the average recovery rate was 100.86%,and RSD was 2.14%（n =6）;the linear range of sinomenine was 0.2 μg ~1.6 μg,the regression equation was Y=18.13X-0.005 5（r=0.999 1）,the average recovery rate was 100.96%,and RSD was 2.34%（n=6）.Conclusion：The methods for identification and content determination have good specificity,repeatability and accuracy and could be used to control the quality of Fengshining capsules.

关 键 词：风湿宁胶囊 薄层鉴别 HPLC 血竭素 青藤碱 

分 类 号：R284[医药卫生—中药学]