硫酸盐还原菌的驯化及硫酸盐降解动力学研究  

Study on the Domestication of Sulphate Reducing Bacteria and the Kinetics of Sulphate Degradation

作　　者：段黎[1] 皮科武[1] 

机构地区：[1]湖北工业大学资源与环境工程学院,湖北武汉430068

出　　处：《湖北工业大学学报》2016年第2期116-120,共5页Journal of Hubei University of Technology

摘　　要：为了有效处理酸性矿山废水(Acid Mineral Drainage,AMD),获得耐低pH的硫酸盐还原菌(Sulphate Reducing Bacteria,SRB),先在pH为7的条件下用Postgate's C培养基对SRB进行富集培养,然后逐步降低培养基中的pH值,依次在pH为6.5、6.0、5.5、5.0、4.5、4.0的Postgate's C培养基中驯化培养SRB,对驯化过程中溶液的pH和硫酸盐浓度进行测定,并对硫酸盐的降解过程进行动力学拟合。结果表明,经驯化后SRB可以在pH为4的环境中生长,驯化传代后的SRB在pH为4的条件下pH上升到5.0左右,硫酸盐的去除率达到38%,驯化效果比较明显。硫酸盐的降解速度模型符合指数速度模型,且培养基中起始pH越低,降解速率越慢。In order to obtain the Sulphate reducing bacteria which can grow under the condition of low pH to deal with low pH acid mine waste water effectively,the SRB was first enriched and cultured on the Postgate＇s C medium at pH 7.Then the pH value of the medium was gradually reduced,and the SRB was respectively acclimated and cultured on Postgate＇s C medium at pH 6.5,6,5.5,5,4.5,4.In the process of domestication,the pH and sulphate concentration were determined,and the kinetics of the degradation of sulphate concentration was fitted.The results showed that the SRB can grow in the environment of pH4.After domestication,the pH increased to about 5under the condition of pH 4,and the removal rate of sulphate reached 38%,and the effect of domestication was obvious.The degradation rate of the sulphate was in accord with the exponential velocity model.The lower the initial Ph is,the slower the degradation rate is.

关 键 词：硫酸盐还原菌 驯化 硫酸盐 降解动力学 

分 类 号：X522[环境科学与工程—环境工程]