血清蛋白质HP、TIMP-1和PEPD与肺结核病理的关联性研究  

Analysis of the association between serum protein levels and the severity of tuberculosis

作　　者：尉理梁[1] 王建华[1] 张秀军[1] 陶学芳[1] 吴玲芳[1] 吕秋琼[1] 张宏梅[2] 冯宪敏[2] 

机构地区：[1]绍兴市第六人民医院呼吸科,浙江绍兴312000 [2]吉林医药学院病原生物学教研室

出　　处：《中国病原生物学杂志》2016年第3期200-203,共4页Journal of Pathogen Biology

基　　金：浙江省医药卫生一般研究计划项目(No.2013KYA209);吉林省教育厅课题(No.2014367)

摘　　要：目的通过临床数据分析和血清蛋白质检测,探索血清蛋白质与肺结核病发病的关系,并通过单/双肺分组,揭示其与疾病严重程度的相关性,阐明其在肺结核病理改变中的作用。方法对94例肺结核病患者和41例健康对照者的临床资料进行分析,同时采用ELISA检测结核病患者和健康对照组的触珠蛋白(haptoglobin,HP)、基质蛋白酶抑制剂-1(tissue inhibitor of metalloproteinases-1,TIMP-1)和脯氨酸肽酶(prolidase,PEPD)。结果肺结核病患者组血清HP、PEPD含量分别为(1 501.05±1 118.91)μg/ml和(1 267.70±1 050.94)mU/ml,健康对照组分别为(814.08±844.76)μg/ml和(863.36±563.29)mU/ml,两组差异均有统计学意义(tHP=2.665,P=0.010<0.01;tPEPD=2.128,P=0.035<0.05)。肺结核病患者单、双肺损害组与健康对照组血清HP含量分别为(1 413.38±994.66)μg/ml、(1 597.08±1 258.93)μg/ml和(814.08±844.76)μg/ml,三组间的差异有统计学意义(F=3.690,P=0.030)。三组血清TIMP-1含量分别为(12 647.99±11 826.39)ng/ml、(26 507.13±18 928.79)ng/ml和(16 742.51±11 569.64)ng/ml,差异有统计学意义(F=3.787,P=0.029);双肺损害组明显高于单肺损害组(t=2.479,P=0.017)。三组血清PEPD含量分别(947.63±540.65)mU/ml、(1 594.89±1 320.46)mU/ml和(863.36±563.29)mU/ml,三组间的差异有统计学意义(F=8.253,P<0.01)。结论肺结核病患者血清HP、TIMP-1、PEPD蛋白与肺结核病发病、病理改变存在关联,血清蛋白质水平可以反应肺结核病的严重程度。Objective Infection with Mycobacterium tuberculosis can activate the innate immune system,disrupt the levels of acute-phase proteins,extracellular matrix proteins,and proteolytic enzymes,and lead to pathological changes such as inflammatory granuloma,caseous necrosis,and cavity formation.The aim of this study is to explore the relationship between the incidence of tuberculosis（TB）and serum proteins by analyzing clinical data and detecting serum proteins to reveal the correlation between serum proteins and the severity of TB in patients with damage to one or both lungs.Methods Clinical data from 94 patients with TB and 41 healthy controls were analyzed using statistical methods.ELISA was used to detect serum levels of haptoglobin（HP）,tissue inhibitor of metalloproteinases 1（TIMP-1）,and prolidase（PEPD）in controls,patients with one affected lung,and patients with both lungs affected. Results Analysis of clinical data showed significant differences in serum albumin,globulin,acute-phase proteins,and lipid metabolism-related proteins（P〈0.01）.Significantly higher levels of serum HP and PEPD（1 501.05±1 118.91μg/ml vs.814.08±844.76μg/ml,tHP=2.665,P=0.010;1 267.70± 1 050.94mU/ml vs.863.36± 563.29mU/ml,tPEPD=2.128,P=0.035）were found in patients with TB in comparison to healthy controls,but the same was not true for TIMP-1（20 079.22 ±17 399.11ng/ml vs.16 742.51± 11 569.64ng/ml,t=0.670,P〉0.05）.Levels of HP,TIMP-1,and PEPD proteins differed in patients with one affected lung,patients with both lungs affected,and healthy controls（1 413.38 ± 994.66μg/ml vs.1 597.08 ± 1 258.93μg/ml vs.814.08 ± 844.76μg/ml,FHP=3.690,P=0.030;12 647.99 ± 11 826.39ng/ml vs.26 507.13 ± 18 928.79ng/ml vs.16 742.51 ± 11 569.64ng/ml,FTIPM-1=3.787,P=0.029;947.63 ±540.65mU/ml vs.1 594.89±1 320.46mU/ml vs.863.36±563.29mU/ml,FPEPD=8.253,P〈0.01）.Significantly higher levels of TIMP-1（26 507.13±18 928.79ng/ml vs.12 647.99±11 826.39ng/ml,t=2.479,P=0.017）were found in patients with both lungs affecte

关 键 词：血清 肺结核病 HP TIMP-1 PEPD 

分 类 号：R521[医药卫生—内科学]