Aβ_(1-40)侧脑室注射模拟阿尔茨海默病树鼩模型的核磁共振成像特征  

Analysis of the MRI characteristics in tree shrew model of Alzheimer's disease induced by intracerebroventricular injection of Aβ_(1-40)

作　　者：郑红[1] 李树德[2] 王振宇[3] 薛整风[4] 张荣平[5] 角建林[1] 

机构地区：[1]昆明医科大学实验动物学部,昆明云南650500 [2]昆明医科大学基础医学院,昆明云南650500 [3]昆明医科大学药学院,昆明云南650505 [4]扬州大学兽医学院,扬州江苏225009 [5]昆明医科大学生物工程中心,昆明云南650031

出　　处：《中国比较医学杂志》2016年第4期1-6,共6页Chinese Journal of Comparative Medicine

基　　金：国家科技支撑计划项目(2014BAI01B01);云南省科技厅应用基础研究联合专项项目(2012FB022);云南省应用基础研究昆医联合专项(2013FB126)

摘　　要：目的分析阿尔茨海默病(AD)模型树鼩大脑影像学特征。方法在脑立体定位仪上侧脑室注射Aβ1-40建立AD动物模型。经视觉-空间行为学检测确定模型成功后,用MRI作脑冠状面T2加权成像(T2WI)和弥散张量成像(DTI)分析。结果模型组模型组参考记忆错误(3周,4周)和工作记忆错误(2周,3周,4周)显著多于对照组(P<0.05)。模型组完成任务的时间(2周,3周)显著多于对照组(P<0.05)。3周起模型组树鼩单侧或双侧海马减小,相应侧脑室或双侧脑室增大。12周时模型组树鼩双侧颞角宽度显著大于对照组和治疗组(P<0.01)。弥散张量成像扫描显示,模型组树鼩海马双侧表观弥散系数(ACD)大于对照组(P<0.01)。模型组胼胝体纤维束缺失严重。结论侧脑室注射Aβ1-40可引起树鼩学习记忆障碍。MRI能显示AD树鼩脑部的特征性改变,颞角宽度、海马ADC值、胼胝体纤维受损对痴呆的诊断有参考价值。Objective To analyze the neuroimaging changes of tree shrew models of Alzheimer＇s disease. Methods Nineteen healthy adult female tree shrews were randomly divided into control（ 5 animals） and model group（ 14 animals）.The model of Alzheimer＇s disease was induced by intracerebroventricular injection of Aβ1- 40 using a stereotaxic devise and proved successfully by visuospatial congnitive task. The in vivo microstructural changes in the brain of tree shrew AD models and control group（ 0,1,2,3,4 weeks） were observed on 1. 5T MRI（ T2WI）,and on 7. 0T MRI（ 12 week）（ T2 WI,DTI）.Results Reference memory errors were increased in the model group at 3 or 4 weeks（ P 0. 05）,and so working memory errors（ P 0. 05） and period of time to perform（ P 0. 05,P 0. 05,P 0. 01） from 2 to 4 weeks. Thus the model was proved to be established successfully. T2 WI test and DTI test were carried out. Hippocampus atrophy of the model group at 3and 4 weeks was observed compared with that at 0 or 1 week or 2 weeks on a 1. 5T Philips Gyroscan. Compared with the control group,the temporal horn width in the model group was significantly increased（ P 0. 01） at 12 weeks on a 7. 0T Bruker Biospec Scanner. DTI test at 12 weeks showed that ADC of bilateral hippocampus was up-regulated in the model group（ P 0. 01）. In the color coded orientation view,loss of the corpus callosum fibers was obvious in the model group.Conclusions Intracerebroventricular injection of Aβ1- 40 can lead to learing and memory impairment in tree shrews. There are abnomal MRI signal changes in the brain,and the temporal horn width,hypocampal apparent diffusion coefficient（ ADC）value and corpus callosum damage may provide reference value for the diagnosis of Alzheimer＇s disease.

关 键 词：阿尔茨海默病 AΒ1-40 树鼩 认知功能 核磁共振成像 

分 类 号：R33[医药卫生—人体生理学]