猕猴全结肠内镜检查方法与观察  

Performance and colonoscopic observation in macaques

作　　者：黄志寅[1] 张琼英[1] 王玉芳[1] 冯哲[1] 赵旭东[2] 吕龙宝[2] 陈文雄[2] 唐川君 龚慧[1] 胡兵[1] 唐承薇[1] 谭庆华[1] 

机构地区：[1]四川大学华西医院消化内科,成都610041 [2]中国科学院昆明动物研究所实验动物中心,昆明650223

出　　处：《中国比较医学杂志》2016年第4期68-71,共4页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金国家(地区)合作与交流项目GZ1065

摘　　要：腹泻是猕猴常见肠道症状,相关肠道病变资料多源于猕猴尸检,尚无成功肠镜检查报告。本文旨在建立猕猴全结肠内镜检查方法及获得猕猴全结肠内镜图像。8只猕猴无〉2月的慢性腹泻史,管喂芒硝后,结肠清洁度符合内镜观察需求。采用电子消化内镜对猕猴全结肠进行观察,操作时间约10~20 min/只。4只动物结肠无明显异常病变,仅部分结构与人类有轻微差异。在4只〈1 d轻泻的猕猴中,内镜观察到结肠小片糜烂及溃疡,其中1只存在明显狭窄。术中及术后1周均无动物死亡。本文建立的猕猴全结肠内镜检查安全有效,对及时诊治猕猴结肠炎、评估药物干预疗效及提供优质实验动物具有重要意义。Diarrhea is a common intestinal symptom in macaque. The corresponding intestinal lesions of macaque are mainly described at autopsy but less observed by colonoscopy. The aim of this study was to develop a colonoscopic technique and to obtain endoscopic images of the entire colon in macaques. Eight healthy adult macaques（ 5 males and 3females） without diarrhea for 2 months,were fed Glauber＇s salt through nasogastric tubes. The colon cleanliness was well matched to the endoscopic observation of macaque colon. The procedure took 10- 20 min for each animal. There was no obvious abnormality in the colon of four animals except some slight differences of mucosal structure from that of human beings. Small pieces of erosion and ulcer in the colons were observed in four macaques which presented mild diarrhea for less than 1 day,while a severe stenosis was observed in one of those four macaques. No animal died during and one week after the endoscopic procedure. Colonoscopy may safely performed in macaques. The images taken by colonoscopy may be important to establish diagnosis and treatment of colitis in macaques in time and to evaluate the efficacy of drug intervention as well. This technique is also helpful to provide qualified macaques for scientific researches.

关 键 词：猕猴 结肠内镜 结肠清洁度 内镜结肠图像 腹泻 

分 类 号：R33[医药卫生—人体生理学]