HPLC法测定盐酸法舒地尔注射液与葡萄糖注射液配伍液中的5-羟甲基糠醛  

Determination of 5-HMF in compatibility solution of Fasudil Hydrochloride Injection and Glucose Injection by HPLC

作　　者：程永科 李锐 陈祖利 

机构地区：[1]河南中帅医药科技股份有限公司,河南郑州451000

出　　处：《现代医药卫生》2016年第9期1317-1319,共3页Journal of Modern Medicine & Health

摘　　要：目的测定盐酸法舒地尔注射液与葡萄糖注射液配伍液中的5-羟甲基糠醛（5-HMF）。方法采用高效液相色谱法（HPLC）,色谱柱：Wetch C18柱（150.0 mm×4.6 mm×5.0μm）,流动相：0.05 mol/L磷酸二氢铵溶液-乙腈（89∶11）,流速：1.0 m L/min,检测波长：284 nm,柱温：25℃,进样量：20μL。结果 5-HMF在1.45-2.61μg/m L内线性关系良好（r=0.999 8）;平均回收率为97.4%（RSD=1.1%）。结论采用HPLC测定盐酸法舒地尔注射液与葡萄糖注射液配伍液中的5-HMF方法简便,准确可靠。Objective To establish a HPLC method for determining the 5-HMF content in the compatibility solution of Fasudil Hydrochloride Injection and Glucose Injection by HPLC. Methods The HPLC method was adopted with the chromato graphic column of Wetch C18column（150 mm×4.6 mm,5 μm）,the mobile phase was 0.05 mol/L ammonium dihydrogen phosphateacetonitrile（89∶11）,the flow rate was 1.0 m L/min,the column temperature was maintained at 25 ℃,the detection wavelength was284 nm,the injection volume was 20 μL. Results 5-HMF had good linear relation in the range of 1.45-2.61 μg/m L（r=0.999 8）.The average recovery rate was 97.4%（RSD=1.1%）. Conclusion Adopting the HPLC method for detecting the 5-HMF content in the compatibility solution of Fasudil Hydrochloride Injection and Glucose Injection is simple,accurate and reliable.

关 键 词：色谱法 高压液相 盐酸 葡萄糖 注射剂 药物配伍禁忌 糠醛/类似物和衍生物 

分 类 号：R541.4[医药卫生—心血管疾病]