荧光定量PCR及酶联免疫吸附试验检测HBV方法比较  

Comparison of FQ-PCR and ELISA for detecting HBV

作　　者：林牧[1] 龚亚东[1] 高绍莹 丁政[1] 马庆庆[1] 

机构地区：[1]贵州航天医院中心实验室,贵州遵义563000

出　　处：《现代医药卫生》2016年第9期1329-1331,共3页Journal of Modern Medicine & Health

摘　　要：目的探讨荧光定量PCR（FQ-PCR）与酶联免疫吸附试验（ELISA）检测乙型肝炎病毒（HBV）的区别,并分析其临床应用价值。方法选取2015年8-12月在该院就诊的221例疑似乙型肝炎患者的血清标本,分别使用FQ-PCR及ELISA法对HBV进行检测,探讨FQ-PCR的检测方法、阳性率及临床意义。结果 FQ-PCR检测共检出134例阳性标本,阳性率为60.63%;ELLSA检测乙型肝炎表面抗原、乙型肝炎e抗原、乙型肝炎核心抗体、乙型肝炎e抗体和乙型肝炎表面抗体阳性率（36.20%、6.33%、33.48%、15.32%、43.90%）均低于FQ-PCR对HBV-DNA检出的阳性率（60.63%）,且分别与大三阳组和小三阳组比较,差异均有统计学意义（P〈0.01）。结论 FQ-PCR能够直观地反映HBV在肝细胞内的复制情况,适合早期诊断并指导临床用药,其诊断和指导意义高于ELISA法,且操作快速简便,值得临床推广。Objective To investigate the difference between fluorescence quantitative PCR（FQ-PCR） and enzyme-linked immunosorbent assay（ELISA） in detecting hepatitis B virus（HBV）,and to analyze their clinical values. Methods The serum samples in 221 cases of suspected hepatitis B in our hospital from August to December 2015 were selected and HBV was respec-tively detected by using FQ-PCR and ELISA for exploring FQ-PCR detection method, positive rate and clinical significance. Results Totally 134 cases of positive samples were detected by FQ-PCR, with the positive rate of 60.63%;the detection positive rates of HBs Ag,HBe Ag,HBc Ab,HBe Ab and HBs Ab by ELISA were 36.20%,6.33%,33.48%,15.32% and 43.90% respectively,which were lower than 60.63% by FQ-PCR,moreover which were compared with the ＂large 3-positve ＂ group and ＂ small 3-positive＂ group,the differences were statistically significant（P〈0.01）. Conclusion FQ-PCR can intuitively reflect the HBV replication situation in liver cells,is suitable for the early diagnosis and guides clinical medication,its diagnosis and guidance significance are higher than those of the ELISA method,moreover its operation is simple and rapid,which deserves to be promoted in clinic.

关 键 词：聚合酶链反应 酶联免疫吸附测定 肝炎病毒 乙型 DNA 病毒 荧光定量PCR 

分 类 号：R512.62[医药卫生—内科学]