重组hIL-10对AA大鼠T淋巴细胞及IL-17A的影响研究  

Effect of recombinant hIL-10 on lymphocytes and IL-17A of an AA rat model

作　　者：杜联峰[1] 孙万邦[1] 丁立珉[1] 唐艳丽[1] 

机构地区：[1]遵义医学院珠海校区/贵州省免疫学研究生教育创新基地,珠海51904

出　　处：《中国免疫学杂志》2016年第4期476-479,共4页Chinese Journal of Immunology

基　　金：贵州省教育厅自然科学重点培育项目(2008032)资助

摘　　要：目的:以重组人白细胞介素-10(human interleukin-10,h IL-10)及其联合甲氨碟呤(MTX)作用佐剂性关节炎大鼠模型,检测T淋巴细胞亚群及IL-17A水平的变化情况,研究重组hIL-10及其联合MTX治疗佐剂性关节炎大鼠的效果。方法:用IL-10、MTX以及IL-10+MTX处理AA大鼠模型,用流式细胞仪测定各组大鼠血液中CD4^+T细胞与CD8^+T细胞比值,酶联免疫吸附试验(ELISA)检测大鼠血清IL-17A的含量。结果:各治疗组CD4^+淋巴细胞总数与CD4^+/CD8^+值不同程度下降,各治疗组与模型组比较有显著性差异(P<0.05)。各治疗组IL-17A水平有不同程度下降,各治疗组与模型组比较有显著性差异(P<0.05),联合组与IL-10组比较有显著性差异(P<0.05)。结论:重组hIL-10能下调AA大鼠血液中CD4^+T细胞的数量及CD4^+/CD8^+比值,降低血清中IL-17A水平,联合组较IL-10组对血清中IL-17A影响更显著,提示重组hIL-10联合MTX在治疗类风湿关节炎中能更好地发挥作用。Objective： Adjuvant arthritis（ AA） rats interfered with recombinant hIL-10,methotrexate（ MTX） separately,we detected the changes of T cell subsets in rat＇s blood and IL-17 A in rat＇s serum. Methods： AA rats interfered with recombinant hIL-10,MTX and IL-10 plus MTX. The changes of the CD4^＋ and CD8^＋T cell subsets were detected by flow cytometry; the Levels of IL-17 A in serum of rats were measured by ELISA method. Results： Compare to normal control group,the CD4^＋T cell total quantity and CD4^＋/CD8^＋T cell Proportion was significantly decreased,there were significant differences between the treatment group and the untreated group（ P〈0. 05）,the IL-17 A of treatment group was significantly decreased,there was a significant differences（ P〈0. 05）. The combination group had significant difference compared with IL-10 group. Conclusion： Recombinant hIL-10 can down-regulate the blood of AA rats in the number of CD4^＋T cells and CD4^＋/ CD8^＋ ratio,reduce serum levels of IL-17 A. The combination group compared with the IL-10 group effect on serum IL-17 A more significantly,the results displayed recombinant hIL-10 plus MTX in the treatment of rheumatoid arthritis can better play the role.

关 键 词：重组人白介素-10 佐剂性关节炎 T细胞亚群 IL-17A 

分 类 号：R392.12[医药卫生—免疫学]