清络通痹方调控miRNA网络干预类风湿关节炎的研究  

Study of Qingluo Tongbi Compound treating rheumatoid arthritis based on miRNA network

作　　者：朱亚梅[1] 周玲玲[1] 彭孝武[1] 耿姗[1] 周学平[1] 

机构地区：[1]南京中医药大学,南京210023

出　　处：《中国免疫学杂志》2016年第4期495-499,共5页Chinese Journal of Immunology

基　　金：国家自然基金项目(81573869);2014江苏省普通高校研究生科研创新计划(SJZZ-0122)资助;南京中医药大学国自然预研基金项目(14XYY01;14XYY10)

摘　　要：目的:观察清络通痹方(QLT)对胶原性关节炎(CIA)小鼠miRNA网络的调控作用,探讨其干预类风湿关节炎的机制。方法:DBA/1小鼠,复制CIA模型,随机分为空白对照、CIA、QLT组,采用miRCURYTM LNA Array芯片技术筛选外周血miRNA异常表达谱,以Realtime PCR对异常变化的miRNAs进行验证,并利用多种生物信息学软件进行分析。结果:与空白对照组比较,CIA小鼠具有差异表达的miRNAs有221个;与CIA比较,QLT差异表达的miRNAs有169个。RT-PCR验证结果与芯片检测所示具有较好的一致性。PCR结果显示CIA中miR-143下调明显,而QLT干预可明显上调miR-143的表达水平。miR-143靶基因显著富集在VEGF、T细胞受体、MAPK信号通路等信号通路中。结论:多种miRNA的异常表达参与CIA的病理过程,QLT可能通过干预miRNA调控网络,多环节、多途径干预RA免疫、炎症、疼痛等多种病理过程,miR-143可能作为QLT治疗RA的重要环节。Objective： To study the mechanism of Qingluo Tongbi Compound（ QLT） treating rheumatoid arthritis（ RA） by observing miRNA Network of QLT on collagen-induced arthritis（ CIA） mice. Methods： The model of RA was induced by collagenⅡ in DBA /1 mice and randomly divided into control group,CIA group,QLT group. Differently expressed miRNAs were detected by miRCURYTM LNA Array. Real-time PCR was applied to verify the reliability of miRNA array. Results： The bioinformatics software and database were applied to predict and analyze target genes. MiRNA array results showed that 221 miRNAs changed in CIA group compared with the control group,and 169 miRNAs changed in QLT group compared with CIA group. And the results of real-time PCR were consistent with the array. Compared with the control group,miR-143 was significantly reduced in CIA group,intervention of QLT obviously upregulated the expression of miR-143. The target genes of miR-143 were significantly stored in VEGF,T cell receptor,MAPK,signaling pathway. Conclusion： Multiple abnormal expression of miRNAs involved in the pathological process of CIA. QLT affected the expression of various miRNAs,which might be related to immunity,inflammation,pain pathological process of RA and miR-143 could be a potential target in the treatment.

关 键 词：MIRNA 胶原性关节炎 清络通痹方 

分 类 号：R285.5[医药卫生—中药学]