不同类型支原体肺炎外周血TIM和TLR基因表达的变化及其意义  

Expression levels of Tim and TLR gene family and its clinical significance in children with different types of mycoplasma pneumonia

作　　者：樊青[1] 颜萍[1] 李培杰[1] 

机构地区：[1]济南市妇幼保健院儿科,济南250001

出　　处：《中国免疫学杂志》2016年第4期546-549,552,共5页Chinese Journal of Immunology

基　　金：山东省济南市卫生科技发展计划项目(2013-33)

摘　　要：目的：探讨不同类型支原体肺炎患儿外周血Toll受体2、4和Tim、1、3家族基因的变化及其意义。方法：随机选取自2013年3月~2014年3月在济南市妇幼保健院儿科住院的支原体肺炎患儿共56例,将入选的56例根据X线影像学改变分为两组：表现为沿支气管分布的结节状、点片状密度增高影或伴网格状、条索状间质改变者作为支气管肺炎组（40例）,表现为节段性、大叶性实变者作为节段性肺炎组（16例）。同时,选取性别和年龄与支原体患儿组匹配的健康查体儿童30例作为对照组。收集外周静脉全血。抽提总RNA,采用qRT-PCR检测TLR-2mRNA、TLR-4mRNA和Tim-1mRNA和Tim-3mRNA的相对表达量,比较各组之间TLR-2mRNA、TLR-4mRNA和Tim-1mRNA和Tim-3mRNA的相对表达量的差异。结果：（1）支原体肺炎组及MP-支气管肺炎组外周血细胞中TLR-2mRNA、TLR-4mRNA和Tim-l mRNA相对表达值显著高于健康对照组。（2）MP-节段性肺炎组TLR-2mRNA、TLR-4mRNA和Tim-1mRNA、Tim-3mRNA的相对表达量的显著高于健康对照组。（3）MP-节段性肺炎组Tim-1mRNA和Tim-3mRNA的相对表达量的显著高于MP-支气管肺炎组。结论：不同类型的支原体肺炎患儿外周血TLR-2、TLR-4和Tim-1基因均有过度表达,Tim-1、Tim-3基因在肺部感染重症患儿有过度表达,TLR2、4受体和Tim1、3家族基因参与不同类型的支原体肺炎的感染过程。Objective： To explore expression levels of Tim and TLR gene family in children with different types of mycoplasma pneumonia and their clinical significance. Methods： 56 patients with mycoplasma pneumonia were randomly selected in Ji＇nan Maternity and Child Care Hospital from March,2013 to March,2014. All the patients were divided into bronchopneumonia group（ n = 40） and segmental pneumonia group（ n = 16） according to their X-ray manifestations. Bronchopneumonia group was characterized as dot,nodular increased density shadow with or without reticular,stripe-like interstitial lesion. Segmental pneumonia was described as segmental or lobar consolidation of lung in X-ray. Meanwhile,30 healthy children were involved in control group with matched age and gender. Peripheral venous blood of all the children in every group was collected and total RNA was extracted. The mRNA of Toll-recepter 2,4 and Tim1,3 was quantified by quantitative real-time PCR（ qRT-PCR）,and compared between different groups. Results： The expression of mRNA of TLR-2,4 and Tim1 significantly increases in both bronchopneumonia group and segmental pneumonia group compared to healthy control. Meanwhile,the mRNA of TLR-2,4 and Tim-1,3 was significantly elevated among segmental pneumonia group compared to healthy control. In addition,the gene expression of Tim1,3 was higher in segmental pneumonia group than bronchopneumonia group. Conclusion： The TLR-2,4 and Tim1 gene are over expressed in both types of mycoplasma pneumonia and Tim-1,3 are highly expressed in children with severe infection in lung. The expression alteration of TLR-2,4 and Tim1,3 gene is involved in different types of mycoplasma pneumonia.

关 键 词：肺炎 支原体 支气管肺炎 节段性肺炎 基因表达 QRT-PCR 

分 类 号：R725.6[医药卫生—儿科]