血小板表面相关抗体检测对ITP的诊断作用研究  

The significance of detection of platelet surface associated antibodies in diagnosis of ITP

作　　者：曾明[1] 程樱 钱婷[1] 侯伶俐[3] 

机构地区：[1]湖北省临床检验中心,武汉市430000 [2]湖北省食品药品监督检验研究院 [3]湖北省十堰市太和医院

出　　处：《河北医药》2016年第10期1494-1497,共4页Hebei Medical Journal

摘　　要：目的研究血小板表面相关抗体检测对特发性血小板减少性紫癜(ITP)的诊断作用研究。方法选取2012年1月至2013年1月收治的ITP患者50例(A组),非免疫性血小板减少患者20例(B组),其他自身免疫性疾病患者20例(C组),另征集20名健康志愿者作为D组。测定4组血小板膜糖蛋白Ⅱb-Ⅲa(PAC-1)以及血小板相关抗体PAIg G、PAIg A、PAIg M水平,对ITP患者进行病情分级,并对治疗后进行疗效评定,统计各个分级的ITP患者及不同疗效患者血小板相关抗体阳性率。结果 4组PLT、PAC-1、PAIg A、PAIg G、PAIg M水平差异有统计学意义(P<0.05)。A组PAIg A、PAIg G、PAIg M均明显高于其他3组患者(P<0.05)。ITP 1、2、3级患者PAIg A、PAIg G、PAIg M阳性率差异有统计学意义(P<0.05)。且随着ITP分级升高,患者PAIg A阳性率呈升高趋势(r值分别为0.712、0.614、0.732,P<0.05)。显效、进步、无效组患者PAIg A阳性率差异有统计学意义(P<0.05),且随着治疗效果的改善,PAIg A阳性率呈降低趋势(r=0.642,P<0.05)。显效、进步、无效组患者PAIg G、PAIg M阳性率差异有统计学意义(P<0.05)。且随着疗效改善,患者PAIg G阳性率呈升高趋势(r值分别为0.723和0.721,P<0.05)。结论 ITP患者血小板表面相关抗体较正常人有所升高,且血小板相关抗体高低与病情程度有一定的相关性,有助于临床ITP的诊疗。Objective To investigate the significance of detection of platelet surface associated antibodies in diagnosis of idiopathic thrombocytopenic purpura（ ITP）. Methods The subjects in the study were divided into four groups,including50 patients who were treated in our hospital from January 2012 to January 2013 in group A,20 patients with non immune thrombocytopenia in group B,20 patients with the other autoimmune diseases in group C,moreover,the 20 healthy subjects were served as control group（ group D）. The levels of platelet membrane glycoprotein Ⅱb-Ⅲa（ PAC-1）,platelet associated antibodies including PAIg G,PAIg A,PAIg M were detected in the four groups,and the pathogenetic condition of patients with ITP was graded. After treatment the therapeutic effects were evaluated,furthermore,the positive rates of platelet surface associated antibodies were statistically analyzed. Results There were significant differences in the levels of PLT,PAC-1,PAIg A,PAIg G,PAIg M among the four groups（ P〈0. 05）. The levels of PAIg A,PAIg G,PAIg M in group A were significantly higher than those in the other three groups（ P〈0. 05）. There were significant differences in the positive rates of PAIg A,PAIg G,PAIg M among patients with ITP at grade 1,2,3（ P〈0. 05）. Moreover with the increase of ITP grading,the positive rates of PAIg A of patients with ITP were increased（ r = 0. 712,0. 614,0. 732,respectively,P〈0. 05）. There were significant differences in the positrive rates of PAIg A among excellence group,improvement group,inefficiency group（ P〈0. 05）,furthermore,with the improvement of therapeutic effects,the positive rates of PAIg A were significantly decreased（ r =0. 642,P〈0. 05）. There were significant differences in the positive rates of PAIg G,PAIg M among excellence group,improvement group,inefficiency group（ P〈0. 05）,furthermore,with the improvement of therapeutic effects,the positive rates of PAIg G were obviously increased（ r = 0. 723,0. 721,respectively,P〈0. 05）. Con

关 键 词：血小板表面相关抗体 ITP PAIgA PAIGG PAIgM 

分 类 号：R554.6[医药卫生—血液循环系统疾病]