丹参多酚酸对抑郁模型大鼠认知功能的改善作用  

Salvianolic acid improves cognitive function in rats with depression

作　　者：贾立娜[1] 于鲁璐[2] 陈欠欠 李云鹏[1] 甄凤亚 卢文婷[1] 荣蓉[1] 王硕[1] 赵栋[1] 许顺江 安翠霞[2] 

机构地区：[1]河北医科大学,石家庄050031 [2]河北医科大学第一医院精神科、河北医科大学精神卫生研究所,石家庄050031 [3]河北省脑老化与认知神经科学重点实验室,石家庄050031

出　　处：《中华行为医学与脑科学杂志》2016年第4期289-294,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然基金面上项目（81271489）;河北省卫计委医学科学研究计划（20130277）;河北省科技厅科技支撑计划（15277713D）

摘　　要：目的探讨丹参多酚酸对抑郁大鼠认知功能障碍的影响。方法92只体质量110～150g清洁级雄性sD大鼠，按随机数字法分为5组，即非应激＋生理盐水组（对照组），n=19，应激＋生理盐水组（生理盐水组，n=19），应激＋氟西汀组（氟西汀组，n=18），应激＋丹酚酸组（丹酚酸组，n=20），应激＋氟西汀＋丹酚酸组（联合组，n=16）。采用慢性温和应激（Chronic mild stress，CMS）建立抑郁大鼠模型，造模成功后，应激各组继续给予应激，且每天腹腔分别注射氟西汀20mg·kg^-1、丹参多酚酸40mg·kg^-1、氟西汀20mg·kg^-1＋丹参多酚酸40mg·kg^-1及等体积生理盐水，体积体质量比10ml·kg^-1。应激3周后、给药3周后测体质量（g）、计算体质量增加值（g）、蔗糖偏好率（％），采用Morris水迷宫实验（Morris water maze，MWM）来评定大鼠认知功能。结果应激3周后：5组大鼠的体质量差异有统计学意义（F=11．828，P〈0．01），对照组的体质量高于应激各组（均P〈0．01）；对照组蔗糖偏好率高于其他组（均P〈0．05）；水迷宫前5d测试显示5组之间差异有统计学意义（F=5．732，P〈0．01），测量时间之间差异有统计学意义（F=136．000，P〈0．01），对照组逃避潜伏期低于其他组（均P〈0．05）。对照组第6天穿越平台象限时间高于其他应激4组（均P〈0．01）。给药3周后：生理盐水组的体质量增加值低于氟西汀、丹酚酸、联合组（均P〈0．01）；氟西汀、丹酚酸、联合组的蔗糖偏好率高于生理盐水组（均P〈0．01）；前5d，5组大鼠MWM逃避潜伏期差异有统计学意义（F=16．243，P〈0．01），测量时间之间差异有统计学意义（F=9．359，P〈0．01）。第6天穿越平台象限时间差异有统计学意义（F=14．499，P〈0．01）。生理盐水组第6天穿越平台象限时间[（9．39±2．22）s]低于对照组[（16．93±4．49）s]，�Objective To investigate the effect of salvianolic acid on cognitive function in rats with depression. Methods 92 SD male rats were randomly divided into 5 groups：non stress ＋ saline （control group, n= 19）, stress＋saline （ saline group, n = 19 ）, stress ＋ fluoxetine （ fluoxetine group, n = 18 ）, stress ＋ salvianolic acid （salvianolic acicl group, n = 20） and stress ＋ salvianolic acid＋ fluoxetine （combination group, n= 16）. Chronic mild stress was used to establish depression model in rats. When models were successfully established, fluoxetine 20 mg ·kg^-1 （ A）, salvianolic acid 40 mg·kg^-1 （ B）, combination of A and B and 0.9% sodium chloride were given by intraperitoneal injection respectively, the volume to body weight ratio was 10 ml·kg^-1. After 3 weeks of stress and drug administration,the added weight （g） and sucrose pref-erence rate （%） were measured. The Morris water maze （MWM） test was adopted after 3 weeks＇ stress and 3 weeks＇ drugs injection to evaluate the change of cognitive function. Results After 3 weeks＇ stress,a significant difference was found among 5 groups （F= 11.828, P〈0.01 ） and the added weight of control group was higher than other groups（ all P〈0.05 ） .There was significant difference of the mean of average escape latency among 5 groups＇ rats during the first 5 days of MWM（F= 5.732, P〈0.01 ）, and difference was found in the measurements of the time （F= 136.000, P〈0.01）. The average escape latency of control group was lower （all P〈0.05） ,and the time of across the platform quadrant were longer than those of other groups （ all P〈0.01 ）. After 3 weeks＇ drug injection,the added weight in saline group was lower than that of the groups of fluoxe- tine,salvianolic acid and combination（ all P〈0.01 ）.The sucrose preference rate in the groups of fluoxetine, salvianolic acid,and combination group was lower than that of saline group（ all P〈0.01 ）.In MWM test,the difference of average avo

关 键 词：丹参多酚酸 抑郁 慢性温和应激 认知功能 水迷宫 

分 类 号：R285.5[医药卫生—中药学]