去氢紫堇碱对小鼠完全弗氏佐剂导致的机械性痛觉过敏的影响  

Effects of dehydrocorydaline on complete Freund＇s adjuvant-induced mechanical hyperalgesia in mice

作　　者：殷智宇[1] 楚帅帅 孙青[1] 李璐[2] 顾小萍[1] 马正良[2] 

机构地区：[1]徐州医科大学&江苏省麻醉学重点实验室,江苏省麻醉与镇痛应用技术重点实验室,徐州221000 [2]南京大学医学院附属鼓楼医院麻醉科,南京210000

出　　处：《中华行为医学与脑科学杂志》2016年第4期300-303,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金（81171048,81171047）;江苏省医学重点学科（XK201140）

摘　　要：目的观察去氢紫堇碱（DHC）对小鼠完全弗氏佐剂（CFA）导致的机械性痛觉过敏的影响。方法采用随机数字表法将40只ICR小鼠分为4组（CFA实验中，实验组8只，对照组8只；自发活动及脏器系数测定实验中，实验组12只，对照组12只），小鼠右后足底皮下注射CFA建立疼痛模型，模型建立第3天实验组腹腔注射10mg／kg DHC，对照组腹腔注射溶媒（10％DMSO），测定给药前及给药后0．5h，1h，2h，3h小鼠机械缩足反射阈值（PWMT）。测定DHC对小鼠自发活动和脏器系数的影响。结果实验组与对照组小鼠的基础PWMT值差异无统计学意义[（10．27±1．34）g vs（10．28±0．35）g，P〉0．05]。与对照组相比，实验组小鼠在给药后0．5h，1h，2h，3h的PWMT升高，差异有统计学意义[0．5h：（8．18±0．87）g vs（4．85±0．65）g；1h：（7．85±0．59）g vs（4．84±0．54）g；2h：（7．36±0．49）g vs（4．90±0．59）g；3h：（6．66±0．45）g vs（5．00±0．36）g；均P〈0．01]。与对照组相比，实验组小鼠2min内穿越格子数及前肢抬起站立次数的差异无统计学意义（P〉0．05），对小鼠肝、肾、脾、心、肺和大脑脏器系数的影响差异无统计学意义（P〉0．05）。结论DHC可以有效缓解小鼠CFA导致的机械性痛觉过敏。Objective To investigate the effects of dehydrocorydaline（DHC） on complete Freund＇s adjuvant （CFA）-induced mechanical hyperalgesia in mice. Methods 40 mice were divided randomly into 4 groups （ CFA test： experiment group = 8, control group = 8 ; locomotor activity and organ coefficient test ： experiment group= 12, control group = 12）. Subcutaneously injected CFA in the plantar of mice to establish pain model. The experimental group mice were injected with 10 mg/kg DHC while the control group mice received 10% DMSO. The paw withdrawal mechanical threshold （PWMT）of mice was tested before and after administration of DHC. The effects of DHC on spontaneous activity and organ coefficient were observed in mice. Results The basic values of PWMT showed there were no statistically significant differences between experimental group and control group （ （ 10.27± 1.34）g vs （10.28±0.35） g, P〉0.05）.Compared with the control group, the values of PWMT in experimental group at 0.5 h, 1 h, 2 h, 3 h after administration of DHC were significantly increased（0.5 h： （8.18±0.87） g vs （4.85±0.65） g; 1 b： （7.85±0.59） g vs （4.84±0.54） g;2 h： （ 7.36±0.49 ） g vs （ 4.90±0.59 ） g; 3 h ： （ 6.66±0.45 ） g vs （5.00±0.36） g; all P〈0.01 ）. Compared with the control group, no significant effect was observed on the number mice crossed grids and lifted forelimb and stood in 2 min in the experimental group （P〉 0.05 ）. And no significant effect was observed on the liver, kidney,spleen,heart,lung and brain organ coefficient in the experiment group （P〉0.05）. Conclusion DHC can alleviate CFA-induced mechanical hyperalgesia in mice.

关 键 词：去氢紫堇碱 完全弗氏佐剂 机械性痛觉过敏 

分 类 号：R402[医药卫生—临床医学]