核黄素荧光猝灭法测定2,4,6-三硝基苯酚  

Fluorescent Quenching Method for the Detection of 2,4,6-Trinitrophenol Using Riboflavin

作　　者：黄小梅[1] 邓祥[1] 

机构地区：[1]四川文理学院化学化工学院,特色植物开发研究四川省高校重点实验室,四川达州635000

出　　处：《应用化学》2016年第5期606-610,共5页Chinese Journal of Applied Chemistry

基　　金：四川省科技厅项目资助(2015JY0033);四川省教育厅重点项目资助(13ZA0101);“特色植物开发研究”四川省高校重点实验室项目资助(sctz201305);四川文理学院重点项目资助(2014Z007Z)

摘　　要：基于核黄素与2,4,6-三硝基苯酚混合后产生荧光猝灭现象,建立了核黄素作为荧光探针测定2,4,6-三硝基苯酚的新方法。在0.2 mol/L磷酸盐（Na H_2PO_4-Na_2HPO_4）缓冲溶液（p H=6.2）中,响应时间为1 min时,检测2,4,6-三硝基苯酚的线性范围为2.5～1000μmol/L,相关系数为0.9938,检测限为0.55μmol/L。当加入5.00和20.00μmol/L 2,4,6-三硝基苯酚到水样后,回收率在98.2%～103.5%之间。方法简便,选择性好,线性范围宽,可用于实际水样中2,4,6-三硝基苯酚的定性定量分析。A novel method for the determination of 2,4, 6-trinitrophenol has been developed based on fluorescent quenching of riboflavin by 2,4,6-trinitrophenol （TNP） in aqueous solutions. The proposed fluorescent quenching method for TNP detection at pH = 6.2 using 0.2 mol/L phosphate （NaH2PO4- Na2HPO4） as buffer solution responses within 1 min and with a broad linear relationship from 2.5 to 1000 umol/L（r =0. 9938）. The limit of detection for TNP is 0. 55 umol/L. When 5.00 and 20. 00 umol/L TNP is added to different water samples, the recovery ranges from 98.2% to 103.5%. Furthermore, this method is simple, selective, and with wide linear range. Therefore, it can be applied in the determination of 2,4,6-trinitrophenol in real samples.

关 键 词：核黄素 三硝基苯酚 荧光猝灭法 

分 类 号：O657.3[理学—分析化学]