异鼠李素抗癌活性研究  

Research on the Antitumor Activity of Isorhamnetin

作　　者：谭琪明[1] 胡丹[2] 

机构地区：[1]铜仁职业技术学院,贵州铜仁554300 [2]贵州省生化工程中心,贵州贵阳550025

出　　处：《当代化工》2016年第4期687-689,共3页Contemporary Chemical Industry

基　　金：贵州省科技计划项目[课题任务书合同编号:黔科合SY字[2013]3014]

摘　　要：为了研究沙棘中异鼠李素的抗癌活性,分别采用了SRB法测试不同浓度异鼠李素对人乳腺癌细胞株MCF-7细胞的抑制率以及MTT法测试不同浓度异鼠李素对人白血病细胞株K562和HL60的抑制率。结果为不同浓度异鼠李素对人乳腺癌细胞株MCF-7细胞的抑制率达(59.42±4.40)%^(69.55±3.17)%,其中0.063μmol/mL异鼠李素对人乳腺癌细胞株MCF-7细胞抑制率为(69.55±3.17)%,与0.3μmol/mL阿霉素相当;不同浓度异鼠李素对人白血病细胞株K562的抑制率达(9.84±1.14)%^(100±4.12)%,其中0.633μmol/mL异鼠李素对人白血病细胞株K562细胞抑制率为(100±4.12)%,与10μmol/mL阿霉素相当;不同浓度异鼠李素对人白血病细胞株HL60的抑制率达(12.50±1.41)%^(100±6.89)%,其中0.633μmol/mL异鼠李素对人白血病细胞株HL60细胞抑制率为(100±6.89)%,与10μmol/mL阿霉素相当。可见异鼠李素具有一定的抗癌活性。The antitumor activity of isorhamnetin was studied. The human breast cancer cell line MCF7 treated with different concentrations of isorhamnetin were examined for cell inhibitory activity by using SRB assay and the human leukemia cell lines K562 and HL60 treated with different concentrations of isorhamnetin were examined for cell inhibitory activity by using MTT assay. The results showed that under the condition of different concentrations of isorhamnetin, the highest human breast cancer cell line MCF7 inhibition rate was（69.55±3.17）%, the lowest was（59.42±4.40）%. The human breast cancer cell line MCF7 inhibition rate of 0.3 μmol/m L adriamycin reached（76.08±3.32）%, there was no significant difference between it and 0.063μmol/mL isorhamnetin. And under the condition of different concentrations of isorhamnetin, the highest human leukemia cell line K562 inhibition rate was（100±4.12）%, the lowest was（9.84±1.14）%. The human leukemia cell line K562 inhibition rate of 10 μmol/mL adriamycin reached（87.59±1.38）%, there was no significant difference between it and 0.633μmol/mL isorhamnetin. And under the condition of different concentrations of isorhamnetin, the highest human leukemia cell line HL60 inhibition rate was（100±6.89）%, the lowest was（12.50±1.41）%. The human leukemia cell line HL60 inhibition rate of 10μmol/mL adriamycin reached（94.20±2.17）%, there was no significant difference between it and 0.633μmol/mL isorhamnetin. Isorhamnetin had a certain activity of antitumor.

关 键 词：异鼠李素 抗癌 活性测定 

分 类 号：TQ460[化学工程—制药化工]