大鼠脑损伤后EPO及其受体表达与损伤时间的关系  

Relationship between Injury Time and Expressions of EPO and Its Receptors in Rats Brain after Cerebral Injury

作　　者：李伟[1] 齐麟[1] 

机构地区：[1]铁道警察学院公安技术系,河南郑州450053

出　　处：《法医学杂志》2016年第2期90-93,共4页Journal of Forensic Medicine

基　　金：公安部科技应用创新项目(2012YYCXTDGZ138)

摘　　要：目的研究大鼠脑损伤后脑组织中促红细胞生成素(erythropoietin,EPO)及其受体(erythropoietin receptor,EPOR)的表达与损伤时间的关系。方法 72只SD大鼠随机分为对照组(36只)、脑损伤组(36只),在建模后1、2、4、8、12、24 h每组各取6只大鼠处死,取挫伤部位脑组织,应用实时荧光定量PCR和Western印迹法检测不同时间点EPO以及EPOR的mRNA表达和蛋白表达情况。结果伤后24h内,EPO及EPOR的表达随损伤时间的增加而呈上升趋势。EPO的mRNA及蛋白表达与损伤时间之间存在相关性,其相关系数分别为0.875、0.911(P<0.05);EPOR的mRNA及蛋白表达与损伤时间之间存在相关性,其相关系数分别为0.936、0.905(P<0.05)。结论大鼠颅脑损伤早期EPO及EPOR的表达呈逐步上升趋势,与损伤时间具有相关性,可以作为脑损伤时间推断的参考。Objective To explore the relationship between injury age and expressions of erythropoietin （EPO） and its receptor EPOR in the brain tissue of rats after cerebral injury. Methods Seventy-two rats were randomly divided into control group （36 rats） and cerebral injury group （36 rats）. The rats were sacrificed at 1, 2, 4, 8, 12, 24 h after cerebral injury （6 rats at each time point） and the brain tissues were extracted. The expressions of mRNA and protein of EPO and EPOR at different time points were detected by real-time fluorescent quantitative PCR and Western bloting. Results The expressions of EPO and EPOR increased within 24 h after injury. The expressions of mRNA and protein of EPO were related to the injury age, and the correlations were 0.875, 0.911, respectively （P〈0.05）. The expressions of mRNA and protein of EPOR were related to the injury age, and the correlation coefficients were 0.936, 0.905, respectively （P〈0.05）. Conclusion The expressions of EPO and EPOR increase gradually in the early stage of the rat＇s cerebral injury, which are associated with the injury age and could be a useful value for estimating injury age.

关 键 词：法医病理学 脑损伤 促红细胞生成素 促红细胞生成素受体 损伤时间 大鼠 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]