两个豚鼠品系速发型哮喘模型中组胺受体的表达及对肺功能的影响  

Histamine Receptor Expression and Their Effect on Pulmonary Function in Immediate Asthma Model of Two Guinea Pig Strains

作　　者：卫振[1] 娄绘芳[1] 吴凯[1] 陈雁虹[1] 谢敏[1] 刘迪文[1] 谢强敏[1] 

机构地区：[1]浙江大学实验动物中心,杭州310058

出　　处：《实验动物与比较医学》2016年第2期101-106,共6页Laboratory Animal and Comparative Medicine

基　　金：浙江省公益性技术应用研究计划（2012C37085）

摘　　要：目的建立Zmu-I：DHP和DHP两个豚鼠品系的速发型哮喘模型，探讨组胺受体I（HIR）和组胺受体2（H2R）在肺组织中的表达及对肺功能的影响。方法两个豚鼠品系分别设置模型组（MW，MP）和对照组（BW，BP），模型组采用卵清白蛋白（OVA）致敏，对照组使用生理盐水。第1，14日致敏豚鼠，第28日用OVA雾化激发，记录0～8min内动态肺顺应性（Cdyn）和气道阻力（Raw）变化。取肺组织样品，进行H1R和H2RmRNA表达检测和免疫组织化学分析。结果OVA雾化激发后，Zmu－1：DHP和DHP品系模型组（MW，MP）Cdyn下降，Raw上升，变化幅度先大后小，约3min达到最大，而两品系对照组（BW，BP）变化较小。其中，MW组反应较MP组更敏感。定量PCR分析，MW模型组的H1R，H2RmRNA表达量高于对照组BW（P〈0．05，P〈0．05），肺组织切片HE染色，模型组（MW，MP）肺泡间可见明显的炎症细胞浸润，而对照组（Bw，BP）未见。免疫组织化学可见，HIR和H2R主要分布在气道和血管周围的平滑肌上。结论速发型哮喘模型，HIR和H2R主要分布在肺部气管周围的平滑肌上，H1R的高表达与哮喘模型的气道敏感性密切相关。Objective To establish immediate asthma model in two strains of guinea pigs （Zmu-1： DHP and DHP） with ovalbumin （OVA）, and find out histamine receptor （H1R, H2R） gene expression difference and their effect on pulmonary function. Methods Two strains of Zmu- 1 ： DHP and DHP guinea pigs were divided into two model groups （MW and MP） and two control groups （BW and BP） respectively. Guinea pigs of model groups were sensitized with OVA, as control groups were treated with normal saline, on the first and 14th day, each guinea pig of model groups were given OVA. On the 28th day,dynamic lung compliance （Cdyn） and airwayresistance （Raw） of every guinea pig were recorded from 0 to 8 minutes after exposure to OVA. Then guinea pigs were sacrificed, lung tissue were examined by HIR, H2R mRNA expression test and immuohistochemistry （IHC） analysis. Results After exposure to OVA, guinea pigs of Zmu-1 ： DHP and DHP model groups （MW and MP） had lower Cdyn and higher Raw changes than control groups （BW and BP） from 0 to 8 minutes and with the maximum Cdyn and Raw change at third minute. Group BW had more sensitive reaction than Group BP. QPCR analysis revealed, group MW had higher mRNA expression of H1R and H2R than group BW （P〈0.05）. Obvious inflammatory cell infiltration of lung tissues were observed by HE staining in model groups （MW and MP）, but didn＇t in control groups （BW and BP）. By IHC, the H1R and H2R expression were observed in smooth muscle of bronchus and vessel. Conclusion In immediate asthma model, H1R and H2R express mainly in smooth muscle of bronchus, its sensitive may have high relation to expression of H1R.

关 键 词：哮喘 卵清白蛋白(OVA) 组胺受体 肺功能 豚鼠 

分 类 号：R562.25[医药卫生—呼吸系统] R-332[医药卫生—内科学]