泡沫法分离苜蓿叶蛋白工艺优化（英文）  

Technology optimization of Medicago sativa leaf protein separation with foam fractionation

作　　者：刘海彬[1] 张炜[1] 陈元涛[1] 高中超[1] 刘龙[1] 

机构地区：[1]青海师范大学化学系,西宁810008

出　　处：《农业工程学报》2016年第9期271-276,共6页Transactions of the Chinese Society of Agricultural Engineering

基　　金：Project supported by the Ministry of science and technology personnel service enterprise(2009GJG20017)

摘　　要：为了研究泡沫分离苜蓿分离的条件,试验在单因素的基础上,采用响应面法对p H值、装液量、料液比、气体流速4个因素进行优化,以富集比和回收率为指标,最佳工艺条件:p H值为7.0、料液比为87.5 mg/L、装液量为600 m L、气体流速为600 m L/min。在此条件下叶蛋白的回收率为90.2%;富集比为7.64。泡沫分离法分离苜蓿蛋白是一种简单、有效的方法,该研究可为苜蓿叶蛋白的深加工提供参考。In order to purify Medicago sativa leaf protein effectively, foam fractionation which could increase both enrichment ratio and recovery of Medicago sativa leaf protein, was developed based on the research of the influences of initial p H value, the air flow rate, the loading liquid volume and the ratio of material to solvent. Then the best single factor was defined which was the loading liquid volume of 550 m L, p H value of 6.5, the ratio of material to solvent of 87.5 mg/L, the air flow rate of 250 m L/min. Based on the single factor experiment, Box-Behnken design was adopted to investigate the influence of four factors： p H value, the loading liquid volume, concentration and the air flow rate on recovery and enrichment ratio of leaf protein from Medicago sativa. Response surface and contour plots were finally graphed with the recovery and enrichment ratio as the response value. The foam fractionation of leaf protein from Medicago sativa was optimized by response surface methodology. The model P-value were both smaller than 0.0001 and the model were goodness-of-fit and the adequacy of the regression model. The optimum conditions for the foam fractionation of leaf protein from Medicago sativa were found to p H value of 6.92, the ratio of material to solvent of 87.5 mg/L, the loading liquid volume of 600 mL, the air flow rate of 200 m L/min. Under the conditions, the recovery of Medicago sativa leaf protein was 92.2% and the enrichment ratio was 8.03. Considering actual process, The optimization processes were p H value of 7.0, the ratio of material to solvent of 87.5 mg/L, the loading liquid volume of 600 m L, the air flow rate of 200 m L/min. The recovery of Medicago sativa leaf protein was 90.2% and the enrichment ratio was 7.64. respectively. The experimental results showed that foam fractionation was an effective method to separate leaf protein from Medicago sativa.

关 键 词：蛋白 工艺 优化 苜蓿 泡沫分离 

分 类 号：O629.73[理学—有机化学]