300株鲍曼不动杆菌琼脂稀释法与纸片扩散法药敏检测结果比较  

Drug Sensitivity Test Results of 300 Strains of Acinetobacter Baumannii detected by Agar Dilution Method and Disc Diffusion Method

作　　者：郭主声[1] 梁思远[1] 林偲思[1] 朱学海[1] 张丽[1] 

机构地区：[1]中山大学附属东华医院检验科,广东东莞523110

出　　处：《现代生物医学进展》2016年第12期2281-2283,2350,共4页Progress in Modern Biomedicine

基　　金：广东省东莞市科技计划医疗卫生类科研项目(20131051010236)

摘　　要：目的:比较琼脂稀释法和纸片扩散法对鲍曼不动杆菌的药敏试验结果。方法:随机挑选的300株鲍曼不动杆菌,检测其标本及科室的分布情况,并采用琼脂稀释法和纸片扩散法检测鲍曼不动杆菌对环丙沙星(CIP)、庆大霉素(CN)、阿米卡星(AK)、头孢他啶(CAZ)、头孢吡肟(FEP)、左氧氟沙星(LEV)、氨苄西林-舒巴坦(SAM)、妥布霉素(TOB)、美洛培南(MEN)、米诺环素(MH)、头孢哌酮-舒巴坦(SCF)11种抗菌药物的敏感性,比较两种检测结果的差异。结果:300株鲍曼不动杆菌主要分布在痰液标本中,共214株,占71.3%,主要来源于ICU 101株(33.7%)及脑外科59株(19.7%)。药敏检测结果显示,两种检测方法所得的SCF和MH的敏感性差异具有统计学意义(P<0.05),其他9种抗菌药物的药敏检测结果差异没有统计学意义(P>0.05)。结论:琼脂稀释法和纸片扩散法对鲍曼不动杆菌药敏试验结果并不完全一致,临床用药时尤其要注意SCF和MH这两种药物药敏结果的可靠性。Objective： To compare the drug sensitivity test results of 300 strains of Acinetobacter baumannii detected by agar dilution method and disc diffusion method. Methods： The distribution of specimens and departments of 300 strains of Acinetobacter baumannii,which were randomly selected, was detected. The sensitivity of Acinetobacter baumannii to ciprofloxacin（CIP）, gentamicin（CN）, amikacin（AK）, ceftazidime（CAZ）, cefepime（FEP）, levofloxacin（LEV）, ampicillin sulbactam（SAM）, tobramycin（TOB）,meropenem（MEN）, minocycline（MH）, cefoperazone-shubatan（SCF） was detected by agar dilution method and disc diffusion method respectively. The difference of the test results was compared between the two methods. Results： 300 strains of Acinetobacter baumannii were mainly in the sputum specimens, totally 214 strains, accounting for 71.3%, which were mainly from the department of ICU 101strains（33.7%） and cerebral surgery 59 strains（19.7%）. Drug sensitivity test results showed that there were statistical significance in the differences of susceptibility to SCF and MH detected by the two methods（P〈 0.05）, but the differences of susceptibility of 9 antibacterials had no statistical significance（P〉 0.05）. Conclusion： The drug sensitivity test results for Acinetobacter baumannii between agar dilution method and disc diffusion method are not the same, we need to notice the reliability of susceptibility results for MH and SCF should be paid more attention to in clinical treatment.

关 键 词：鲍曼不动杆菌 药物敏感性试验 琼脂稀释法 纸片扩散法 

分 类 号：R378[医药卫生—病原生物学] R969.4[医药卫生—基础医学]