头针对急性脑梗死大鼠不同时间窗Bax与Bcl-2影响的研究  

Bax and Bcl-2 mRNA Efficacy of Scalp Acupuncture on Treating Acute Cerebral Infarction in Rats at Different Time Points

作　　者：李晓宁[1] 迟蕾[2] 吴磊[2] 师帅[1] 

机构地区：[1]黑龙江中医药大学第二附属医院,黑龙江哈尔滨150001 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《针灸临床杂志》2016年第4期73-76,97,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：黑龙江省自然科学基金面上项目;编号:H201466;哈尔滨市科技局科技创新人才研究专项资金项目;编号:2015RAQYJ079;黑龙江中医药大学校基金;编号:201113

摘　　要：目的:观察头针电刺激对不同时间点脑梗死大鼠Bax与Bcl-2 mRNA的变化,探讨其对脑梗死可能作用机制。方法:采用线栓法制作大鼠局灶性脑梗死模型。将180只健康Wistar雌性大鼠,随机分为3组,头针电刺激组、模型对照组和假手术组。各组又依据脑梗死后治疗时间点不同分为4个亚组,即1天组、3天组、7天组与14天组。3组大鼠造模结束24 h起,给予头针电刺激组大鼠针刺百会穴与大椎穴,并进行电刺激治疗,1次/日,30 min/次,余2组在每天相应时间固定大鼠于针刺台,不予任何处置。疗程治疗结束后,断头取脑。运用RT-PCR检测梗死脑组织Bax与Bcl-2 mRNA。3组大鼠在造模结束清醒后、每日治疗前后均要进行神经功能缺损程度评分,采用Zea-longa 5级评分标准。结果:头针电刺激组中7天与14天的亚组大鼠Zea-longa 5级评分明显低于同组1天与3天的亚组(P<0.05),14天的Zea-longa 5级评分与模型组比较,具有显著性差异(P<0.05);头针电刺激组经RTPCR检测后,Bax mRNA的表达逐渐降低,Bcl-2逐渐增加,且14天的亚组与模型组14天比较,有显著性差异(P<0.05),与假手术组14天比较则无显著性差异(P>0.05)。结论:头针电刺激能明显降低脑梗死大鼠的神经功能缺损程度评分,改善大鼠运动功能;头针电刺激能降低Bax mRNA的表达,提高Bcl-2 mRNA的表达,从而抑制缺血区细胞凋亡,保护脑组织,促进肢体康复的功效,其中以头针电刺激14天疗效最佳。Objective： To observe the effect of scalp electrical stimulation at different time points on Bax and Bcl- 2 mRNA changes in cerebral infarction rats,and to explore its possible mechanism. Methods： The rat models of focal cerebral infarction were induced by suture method. The 180 healthy female wister rats were randomly divided into three groups as a scalp electrical stimulation group,a model control group and a sham group. Each group based on the treatment time point after cerebral infarction was divided into four subgroups,namely 1 d group,3 d group,7 d group and 14 d group. Since the end of 24 h of rats modeling in three groups,give scalp electric acupuncture at Baihui and Dazhui in stimulation group,with electrical stimulation therapy,1 time / day,30 min / time,but rats in other two groups in the corresponding time every day were fixed at acupuncture units,without any disposal. After the appropriate course of treatment,the brains were removed. Use RT- PCR to detect Bax and Bcl- 2 mRNA of infarcted brain tissue. After rats were sober at the end of modeling in three groups,neurological impairment score before and after the daily treatment was carried out,using Zea- longa five scoring criteria. Results： Zea- longa five scores in the 7 d and 14 d of the subgroups of scalp electrical stimulation group were significantly lower than those in the 1 d and 3 d subgroups（ P〈0. 05）,Zea- longa five scores in 14 d group had a significant difference compared with the model group（ P〈0. 05）; in scalp electrical stimulation group after RT- PCR the expression of Bax mRNA was gradually decreased,but Bcl- 2 was gradually increased,and the 14 d sub- group was compared with the 14 d model group,with significant differences（ P〈0. 05）,but compared with the 14 d sham group,there was no significant difference（ P〈0. 05）. Conclusion：Scalp electrical stimulation can significantly reduce neurological deficit scores of infarction rats,and can improve motor function in rats; scalp electrical stimulation can red

关 键 词：脑梗死 头针电刺激 Zea-longa评分 BAX BCL-2 

分 类 号：R245.321[医药卫生—针灸推拿学]