脊髓康对脊髓损伤大鼠Nogo-66受体NgR表达的影响  

Effect of Jisuikang on Nogo-66 receptor Ng R expression in rats with spinal cord injury

作　　者：郭杨[1] 马勇[1,2] 冯诚[1] 潘娅岚 黄桂成[1] 

机构地区：[1]南京中医药大学骨伤研究所骨伤修复与重建新技术实验室,江苏省南京市210023 [2]南京中医药大学附属医院骨伤科,江苏省南京市210029

出　　处：《中国组织工程研究》2016年第18期2622-2627,共6页Chinese Journal of Tissue Engineering Research

基　　金：江苏省高校自然科学研究面上项目(15KJD360001);国家自然科学基金项目(81573997);江苏省普通高校研究生科研创新计划项目(CXZZ12_0609);江苏省自然科学基金资助项目(BK2011180)~~

摘　　要：背景:研究发现,脊髓损伤后髓鞘相关抑制分子的产生是影响轴突再生微环境的重要原因。中医药具有多因素、多靶点的优点,正逐步成为改善神经再生微环境的研究热点。目的:探索中药脊髓康对脊髓损伤后Nogo-66受体Ng R表达的影响。方法:144只大鼠随机等分为6组,假手术组、模型组、强的松组、脊髓康高、中、低剂量组,每组24只。后5组以改良Allen’s法建立脊髓损伤动物模型。强的松组大鼠每天给予0.06 g/kg醋酸泼尼松灌胃,1次/d。脊髓康低中高剂量组大鼠每天给予12.5,25,50 g/kg脊髓康灌胃,1次/d。假手术组和模型组大鼠每天给予20 m L生理盐水灌胃,1次/d。各组动物均连续给药至动物处死。结果与结论:与模型组相比,强的松组及脊髓康低中剂量组大鼠脊髓组织中Ng R蛋白及m RNA表达水平均显著降低。提示脊髓康可促进大鼠脊髓损伤后神经功能的恢复,可有效抑制脊髓损伤区Ng R蛋白的表达,从而抑制不利于神经再生的因素,进一步改善神经再生的微环境。BACKGROUND： Myelin sheath related inhibitors have been found to have great impact on microenvironment of axon regeneration. Traditional Chinese medicine is gradual y becoming a research hotspot on improving microenvironment of nerve regeneration with its advantage on multiple factors and targets. OBJECTIVE： To clarify the effect of Jisuikang on Nogo-66 receptor NgR expression after spinal cord injury. METHODS： 144 rats were randomly divided into six groups： sham surgery group, model group, prednisone group, high-, moderate- and low-dose Jisuikang groups （n=24）. Animal models of spinal cord injury were established by the modified Allen’s method in the later five groups. Rats in the prednisone group were daily given 0.06 g/kg prednisone acetate by lavage, once a day. Rats in the high-, moderate- and low-dose Jisuikang groups were daily intragastrically given 12.5, 25 and 50 g/kg Jisuikang, once a day. Rats in the sham surgery and model groups were intragastrically daily given 20 mL of saline, once a day. Rats in each group were administered drugs until death. RESULTS AND CONCLUSION： Compared with the model group, NgR protein and mRNA expression levels were significantly lower in the prednisone, moderate-and low-dose Jisuikang groups. These data suggested that Jisuikang can improve the recovery of neurological function after spinal cord injury and effectively inhibit NgR protein expression at the site of injury so as to suppress the microenvironment factors harmful to nerve regeneration and further improve the microenvironment of nerve regeneration. Subject headings： Drugs, Chinese Herbal; Spinal Cord Injuries; Axons; Tissue Engineering

关 键 词：实验动物 神经损伤与修复动物模型 脊髓康 脊髓损伤 NGR 轴突生长抑制因子 神经再生 微环境 中医药 肾督同治 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]