原花青素对离体肺动脉血管张力的影响(英文)  

Vasorelaxant effects of procyanidins on pulmonary artery in vitro

作　　者：牛彩琴[1] 孙安 雷枭[1] 张团笑[3] 

机构地区：[1]川北医学院中西医临床医学系,四川省南充市637007 [2]解放军兰州军区空军机关医院,甘肃省兰州市370020 [3]川北医学院生理学教研室,四川省南充市637007

出　　处：《中国组织工程研究》2016年第18期2668-2676,共9页Chinese Journal of Tissue Engineering Research

基　　金：the Key Program of Sichuan Department of Education;No.14ZA0182~~

摘　　要：背景:原花青素是广泛存在于植物界中的一类多酚化合物,由不同数量的儿茶素或表儿茶素缩合而成,有保护血管内皮、清除自由基、抗血小板聚集、降低毛细血管通透性、起到降低血压、抗氧化活性、抗水肿、防止冠状动脉粥样硬化性心脏病和动脉粥样硬化等作用。目的:观察原花青素对肺动脉环的舒张作用及机制。方法:家兔离体肺动脉环为标本,以去甲肾上腺素(1×10-6mol/L)预收缩肺动脉环后给予0.625,1.25,2.5 mg/L原花青素观察其张力变化。再将不同浓度原花青素干预的家兔离体肺动脉平滑肌去除血管内皮、给予一氧化氮合酶抑制剂左旋硝基精氨酸(1×10-4mol/L)、甲烯蓝(1×10-5mol/L)、环氧酶抑制剂吲哚美辛(1×10-5mol/L)和β肾上腺素能受体阻断剂普萘洛尔(1×10-5mol/L),探讨它们对血管张力的影响。结果与结论:1原花青素对肺动脉环静息张力无明显影响,但不同剂量的原花青素可使1×10-6mol/L去甲肾上腺素预收缩血管产生明显舒张,并呈量效依赖性(r=0.69,P<0.001)。2去除血管内皮、左旋硝基精氨酸和甲烯蓝均可减弱原花青素舒张血管的作用,但吲哚美辛和普萘洛尔无影响。320 mg/L原花青素能明显压低去除内皮细胞肺动脉血管的去甲肾上腺素、KCl和Ca Cl2的量效收缩反应曲线,且去甲肾上腺素、KCl和Ca Cl2的亲和力指数降低(P<0.01)。4原花青素能抑制去甲肾上腺素引起的第Ⅰ时相收缩,而对Ca Cl2引起的第Ⅱ时相收缩无影响。5实验结果表明原花青素对肺动脉的舒张效应是内皮依赖性的,与NO/c GMP介导有关,可拮抗受体依赖性钙通道和电压依赖性钙通道而降低细胞内Ca2+引起血管舒张。BACKGROUND： Procyanidins is a kind of polyphenol compounds in regnum vegetable, which is composed of different quantities of catechin and epicatechin. Studies show that procyanidins plays a role on protecting vascular endothelium, scavenging free radicals, resisting platelet aggregation, and reducing capillary permeability. Thus, procyanidins has obviously functions of reducing blood pressure, anti-oxidant activity, anti-edema, preventing coronary heart disease and atherosclerosis. OBJECTIVE： To study the vasorelaxant effect of procyanidins on pulmonic rings and its mechanisms. METHODS： Rabbit thoracic pulmonary arteries were isolated. Pre-contracted with noradrenalin （1×10-6 mol/L） and their responses to different concentrations of procyanidins （0.625, 1.25, 2.5 mg/L） were investigated. After removal of the endothelium of pulmonary artery smooth muscle, the effects of different signaling pathway inhibitors on procyanidins-induced relaxation, including nitric oxide synthase inhibitor Nω-Nitro-L-arginine （1×10-4 mol/L）, methylene blue （1×10-5 mol/L）, prostaglandin synthase inhibitor indomethacin （1×10-5 mol/L） and blockage of the adrenergicβ-receptor propranolol （1×10-5 mol/L）, were also assessed. RESULTS AND CONCLUSION： （1） Procyanidins did not change the resting tension of rabbit’s pulmonic rings, but caused an obvious dose-dependent relaxation in 1×10-6 mol/L noradrenalin-precontracted pulmonic rings （r=0.69, P 〈 0.001）. （2） The relaxant effect of procyanidins was significantly reduced by removal of endothelium or by treatment with either Nω-Nitro-L-arginine or methylene blue, but not by treatment with prostaglandin synthase inhibitor or blockage of the adrenergic β-receptor. （3） Procyanidins （20 mg/L） dropped the dose-effect curves of noradrenalin, KCl and on pulmonic rings denuded endothelium. Moreover, affinity index of noradrenalin, KCl and CaCl2 decreased （P 〈 0.01）. （4） Procyanidins also inhibited the vasoconstriction caused by

关 键 词：实验动物 心肺损伤与修复动物模型 原花青素 血管 离体 家兔 肺动脉环 一氧化氮 血管张力 钙离子 

分 类 号：R318[医药卫生—生物医学工程]