口腔软组织缺损兔模型的构建  

Establishment of a rabbit model of oral soft tissue defects

作　　者：杨琴秋[1] 董露[2] 肖琼 陈红亮[4] 孙勇[4] 

机构地区：[1]遂宁市第一人民医院口腔科,四川省遂宁市629000 [2]凉山彝族自治州第一人民医院口腔科,四川省西昌市615000 [3]重庆拜博口腔医院管理有限公司九龙坡口腔医院,重庆市400000 [4]解放军成都军区机关医院口腔科,四川省成都市610041

出　　处：《中国组织工程研究》2016年第18期2684-2689,共6页Chinese Journal of Tissue Engineering Research

基　　金：成都军区"十二五"科研面上项目(C14050)~~

摘　　要：背景:在口腔种植临床,对术区软组织缺损修复方法及材料的研究一直在深入。而通过建立可靠的实验动物口腔软组织缺损模型,会对该领域的探索研究产生积极的深远影响。目的:实验旨在建立一种兔口腔软组织缺损动物模型,以供对口腔软组织缺损修复治疗进行深入的研究。方法:在18只3月龄雄性新西兰兔的硬腭前部中份,分别距上颌门齿、左右硬腭黏膜边缘2 mm处以直径为5 mm的组织环切钻制备圆形软组织全层缺损。分别于建模后3,7,14,21,28,56 d对创面进行形态学和组织学观察。结果与结论:1创面形态:建模后3,7 d,创口炎症反应明显;随着时间推移,炎性反应消退,伤口逐渐愈合。在建模后21,28,56 d可见明显瘢痕形成;2创面组织学变化:在伤后3,7 d,炎细胞大量浸润,组织坏死面积较大,建模后7 d后,随着结缔组织增殖和新生毛细血管的广泛形成,伤口逐渐塑形完整;3结果说明:实验成功构建口腔软组织缺损模型兔,符合创伤愈合的生理规律和人类口腔软组织缺损愈合特点。BACKGROUND： In clinical, the research on the method and the material of the soft tissue defect of the operation area has been in depth. It wil have a positive impact on our exploration and research in this field through the establishment of reliable experimental animal oral soft tissue defect model. OBJECTIVE： To establish a rabbit model of oral soft tissue defects for oral treatment of soft tissue defects in-depth study. METHODS： Eighteen 3-month-old male New Zealand rabbits were enrol ed. A tissue ring cutting dril of 5 mm diameter was used to prepare round ful -thickness soft tissue defects in the front part of the hard palate and, respectively, from the back of the maxil ary incisors, about 2 mm from the hard palate mucosal edge. Morphology and histology were observed at 3, 7, 14, 21, 28 and 56 days after model establishment. RESULTS AND CONCLUSION： （1） Morphological observation： After 3 and 7 days, the wound’s inflammatory reaction was obvious. As the time goes, the inflammatory response subsided, the wound gradual y healed. Scar formation was observed at 21, 28 and 56 days after surgery. （2） Histological observation： 3 and 7 days after injury, many inflammatory cel s were infiltrating, and tissue necrosis area was larger. At 7 days after surgery, with the extensive formation of connective tissue proliferation and new blood capil aries, the wound gradual y shaping completely. （3） Results indicated that a rabbit model of oral soft tissue defects was successful y established, which was in line with the physical laws of wound healing and the characteristics of human oral soft tissue defects healing.

关 键 词：实验动物 口腔损伤与修复动物模型 软组织缺损 创伤愈合 新西兰兔 硬腭 苏木精-伊红染色 组织构建 

分 类 号：R318[医药卫生—生物医学工程]