玻璃体腔注射促红细胞生成素保护糖尿病视网膜病变模型大鼠的视神经作用  

Vitreous cavity injection with erythropoietin protects optic nerve in a rat model of diabetic retinopathy

作　　者：张永红[1] 张晓湄[1] 韦笑一[1] 唐柳苹[1] 刘代华[2] 凌宇[1] 

机构地区：[1]广西壮族自治区柳州市人民医院眼科,广西壮族自治区柳州市545006 [2]广西壮族自治区柳州市人民医院药剂科,广西壮族自治区柳州市545006

出　　处：《中国组织工程研究》2016年第18期2690-2696,共7页Chinese Journal of Tissue Engineering Research

基　　金：广西自然科学基金项目(2010GXNSFB013090)~~

摘　　要：背景:糖尿病会导致多种并发症的出现,其中视网膜病变是一种常见的类型,为探究促红细胞生成素在糖尿病视网膜病变中的作用,建立具有临床视网膜新生血管相似病理特征且重复性高的动物模型是十分必要的。目的:观察玻璃体腔注射促红细胞生成素对糖尿病视网膜病变大鼠视神经的保护作用。方法:建立糖尿病视网膜病变模型大鼠,于玻璃体腔注射促红细胞生成素干预,连续注射14 d,设模型组和正常对照组作对比。结果与结论:1透射电镜观察:与模型组相比,促红细胞生成素组视网膜神经节细胞核呈现出均匀的染色,细胞膜完整,内质网呈轻微肿胀,细胞核周围线粒体存在数量较少的空泡;2Western blot和RT-PCR检测:与模型组相比,促红细胞生成素组Caspase-3激活型、Bax蛋白表达降低(P<0.05),Caspase-3原型蛋白和Bcl-2蛋白表达明显提高(P<0.05),两组核转录因子κB和Survivn基因的表达也差异有显著性意义(P<0.05);3结果证实:玻璃体腔注射促红细胞生成素对糖尿病视网膜病变大鼠视神经有保护作用,可能与其抑制细胞凋亡有关。BACKGROUND： Diabetes can lead to many complications. Of them, retinopathy is a common type. To explore the effect of erythropoietin in diabetic retinopathy, it is necessary to establish an animal model of similar pathologic features and high reproducibility in clinical retinal neovascularization. OBJECTIVE： To investigate the protective effect of vitreous cavity injection of erythropoietin on optic nerve of rats with diabetic retinopathy. METHODS： Rat models of diabetic retinopathy were established and injected with erythropoietin through vitreous space for 14 consecutive days. Results were compared between the model and normal control groups. RESULTS AND CONCLUSION： （1） Transmission electron microscope： compared with the model group, retinal ganglion cel nucleus presented uniform red; cel membrane was intact; endoplasmic reticulum exhibited slight swel ing. A smal number of vacuoles were found in mitochondria around the nuclei. （2） Western blot assay and RT-PCR： compared with the model group, Caspase-3 and Bax protein expression was lower in the erythropoietin group （P 〈 0.05）. Caspase-3 protein and Bcl-2 protein expression was significantly higher in the erythropoietin group than in the model group （P 〈 0.05）. Significant differences in nuclear factor κB and Survivn gene expression were detected between the model and erythropoietin groups （P 〈 0.05）. （3） Results verified that vitreous cavity injection of erythropoietin had neuroprotective effect on rats with diabetic retinopathy, which may be associated with the inhibitory effect on apoptosis.

关 键 词：实验动物 细胞损伤与修复模型 糖尿病 并发症 玻璃体 玻璃体腔 腔内注射 视网膜病变 促红细胞生成素 大鼠 细胞凋亡 凋亡因子 广西壮族自治区自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]