玛咖对力竭运动后老年模型大鼠肾脏线粒体呼吸功能及抗衰老能力的影响  

Effects of Maca on kidney mitochondrial respiratory function and anti-aging capabilities in aged rats after exhausted exercise

作　　者：彭丽娜[1] 贾鹏[1] 

机构地区：[1]廊坊师范学院体育学院,河北省廊坊市065000

出　　处：《中国组织工程研究》2016年第18期2711-2716,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:研究表明,玛咖可增强细胞的免疫功能,提高线粒体呼吸链酶活性,起到抗氧化抗衰老的作用。目的:观察玛咖对力竭运动后老年大鼠肾脏线粒体呼吸功能及抗衰老能力的影响。方法:将10月龄老年大鼠每日灌胃1次玛咖粉剂(5 g/kg),同时进行递增负荷跑台运动,每周5 d,同时设对照组、玛咖组和运动组作对照。训练6周后,各组大鼠均进行力竭运动(35 m/min),随后即刻腹腔注射2%戊巴比妥钠麻醉大鼠取出肾脏,差速离心提取线粒体,分光光度计测定线粒体呼吸链酶活性。结果与结论:1线粒体呼吸链酶及抗氧化酶活性:与对照组、玛咖组和运动组相比,玛咖+运动组线粒体呼吸链酶Ⅰ-Ⅲ、超氧化物歧化酶和谷胱甘肽过氧化物酶活性均升高(P<0.05或P<0.01)、丙二醛含量均降低(P<0.05或P<0.01);2结果说明:补充玛咖结合递增负荷运动训练均可提高力竭运动后老年大鼠肾脏线粒呼吸功能,延缓衰老,且补充玛咖与递增负荷运动具有协同效应。BACKGROUND： Studies have shown that Maca can enhance immune cel function, improve mitochondrial respiratory chain enzymes, and play a role in anti-oxidation and anti-aging. OBJECTIVE： To observe Maca effects on kidney mitochondrial respiratory function and anti-aging capabilities in elderly rats after exhausted exercise. METHODS： 10-month-old elderly rats were intragastrical y administrated Maca powder 5 g/kg, once a day, and did treadmil exercise, 5 days in a week. Rats were randomly divided into control group, Maca group and training group and Maca＋training group. At 6 weeks after training, rats in each group did exhausted exercise （35 m/min）, and immediately received intraperitoneal injection of 2% sodium pentobarbital. The kidney was obtained, and mitochondria were extracted by differential centrifugation. A spectrophotometer was used to measure mitochondrial respiratory chain activity. RESULTS AND CONCLUSION： （1） Mitochondrial respiratory chain enzyme and antioxidant enzyme activity： mitochondrial respiratory chain enzyme I-III, superoxide dismutase and glutathione peroxidase activities were higher in the Maca＋training group than in the control group, Maca group and training group （P 〈 0.05 or P 〈 0.01）, but malondialdehyde content was lower （P 〈 0.05 or P 〈 0.01）. （2） Results suggested that the combination of supplement Maca and incremental exercise can improve mitochondrial respiratory function and delay aging in the kidney of aged rats after exhausted exercise. Moreover, supplemented Maca and incremental exercise have a synergistic effect.

关 键 词：实验动物 运动系统动物模型 玛咖 递增负荷 力竭 运动 老年 大鼠 肾脏 线粒体 呼吸链酶 抗衰老 

分 类 号：R318[医药卫生—生物医学工程]