基于深度测序技术的RNA编辑调控研究  

Understanding RNA Editing Regulation in the Deep Sequencing Era

作　　者：杨欣壮[1] 丁晚秋 陈加余 李川昀[1] 

机构地区：[1]北京大学分子医学研究所,北京100871

出　　处：《生命科学仪器》2016年第1期3-11,共9页Life Science Instruments

摘　　要：RNA编辑是一种重要的转录后修饰,可以导致成熟RNA不同于模板DNA序列。长期以来,受鉴定策略等因素限制,该领域研究举步维艰。近年来,随着深度测序技术的兴起,RNA编辑领域迎来了前所未有的机遇,在全基因组水平上实现了对多物种RNA编辑位点的鉴定,并对其整体的生物学功能开展了初步的探讨。本文就近年来利用深度测序技术对RNA编辑的鉴定、调控元件的特征分析以及RNA编辑功能研究等方面的进展进行综述。RNA editing is a post-transcriptional modification that introduces discrepancies between RNA and its corresponding DNA sequence. For a long time, it is challenging to decipher the RNA editome due to technical limitations. Recently, the deep sequencing technology dramatically accelerated the investigation of the RNA editing regulation, which facilitates the genome- wide＇identification of RNA editome in various species at relatively low cost. Here we will briefly review the recent progress on the identification and functional studies of RNA editing regulation in the deep sequencing era.

关 键 词：深度测序 RNA编辑 编辑组 ADAR酶 分子演化 

分 类 号：Q752[生物学—分子生物学]