嗜麦芽窄食单胞菌全菌灭活苗在小鼠体内的免疫保护作用  

Evaluation of immune protection by inactivated whole bacteria vaccine of Stenotrophomonas maltophilia

作　　者：钱垚[1,2,3] 尚学义[2] 汤雪萍[2] 许广杨 姜永强[3] 李艳[1,2] 

机构地区：[1]安徽医科大学解放军307医院临床学院,合肥230032 [2]军事医学科学院附属医院重症医学科,北京100071 [3]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071

出　　处：《军事医学》2016年第4期316-318,共3页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81071399;81271887;81571959);首都医学发展科研基金资助项目(2009-3071)

摘　　要：目的评价嗜麦芽窄食单胞菌全菌灭活苗在小鼠体内的免疫保护性。方法利用甲醛制备嗜麦芽窄食单胞菌全菌灭活苗并免疫小鼠,检测其抗体效价达到要求后,通过体外健康人血调理杀伤实验以及体内攻毒实验评价全菌灭活苗的保护作用。结果在第二次加强免疫后,免疫组血清中特异性Ig G滴度显著增加,其抗血清在体外具有明显的调理杀伤能力;在体内的攻毒实验中也具有显著的保护作用。结论灭活的嗜麦芽窄食单胞菌全菌灭活苗可刺激诱导动物产生体液免疫应答,使小鼠产生有效的免疫保护作用,为进一步探讨嗜麦芽窄食单胞菌全菌灭活疫苗奠定了良好的实验基础。Objective To evaluate the immunoprotection by the inactivated whole bacteria（ IWB） of Stenotrophomonas maltophilia K279 a in mice. Methods Mice were immunized by inactivated whole bacteria of S. maltophilia K279 a made from formaldehyde. When the indicated the antibody titer of the mice reached the require level,the protective effect of the IWB was evaluated by performing the opsonophagocytic killing test in vitro and the poison attack experiments in vivo.Results It was found that Ig G in serum of the immunized mice measured by ELISA was significantly increased after the second immune enhancement,and antiserum in vitro had strong phagocytic effect. Meanwhile,immunoprotection of the immunized groups was also significantly increased when challenged by S. maltophilia K279 a. Conclusion Effective humoral immune response can be predominantly induced by the inactivated whole bacteria of S. maltophilia K279 a,providing protection against challenge by S. maltophilia K279 a in BALB / c mice.

关 键 词：嗜麦芽窄食单胞菌 全菌疫苗 免疫保护 

分 类 号：R392-33[医药卫生—免疫学]