响应面法优化博落回根生物碱的提取工艺  

Optimization of Extraction Process Conditions of Alkaloids from Macleaya Cordata Roots by Response Surface Methodology

作　　者：陶志杰[1] 王改玲[1] 王永斌[1] 孙晓侠[1] 吴珊珊[1] 

机构地区：[1]蚌埠学院生物与食品工程系,安徽蚌埠233030

出　　处：《蚌埠学院学报》2016年第2期12-14,共3页Journal of Bengbu University

基　　金：安徽高校省级自然科学研究项目(KJ2013Z198)

摘　　要：以博落回根生物碱得率为研究指标,在单因素实验的基础上,采用三因素三水平Box-Behnken中心组合设计和响应面法分析,研究了博落回根生物碱的最佳提取工艺条件。结果表明:超声波功率20 W、料液比1∶13.04(g/m L)、乙醇浓度84.44%、提取时间79.76 min,博落回根生物碱得率预测值为2.86%。在此实际条件下,博落回根中生物碱的平均提取率为2.92%,与理论预测值较为接近,其相对误差为2.05%。Taking Macleaya cordata roots as the research object,the optimal extraction process was determined using central composite design and response surface methodology based on single factor investigations. The results showed that the optimum ultrasonic-assisted conditions were ultrasonic power 20 W,ratio of liquid to solid 1∶ 13. 04（ g / m L）,84. 44% ethanol,extracting time 79.76 min,the alkaloids extraction rate would be 2. 89%. The average actual yield of alkaloids was 2. 92%. Compared to the theoretical value,the relative error was 2. 05%.

关 键 词：博落回 生物碱 超声萃取 响应面法 

分 类 号：R284.2[医药卫生—中药学]