绝经后妇女宫颈糜烂组织hTERC基因表达的临床意义  

Clinical significance of hTERC expression in post-menopausal women with cervical erosion

作　　者：彭湃[1] 段清[1] 魏殿军[1] 

机构地区：[1]天津医科大学第二医院检验科,天津市300211

出　　处：《实用检验医师杂志》2016年第1期10-13,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的探讨绝经后妇女宫颈糜烂组织中人类染色体末端酶(human telomerase RNA component,hTERC)基因的表达及其临床意义。方法选择2015年2月至2015年8月于我院就诊的绝经1年及以上妇女经肉眼观为宫颈糜烂的患者60例,根据宫颈糜烂程度分为宫颈轻度糜烂组(19例)、中度糜烂组(23例)、重度糜烂组(18例)。另收集同期健康查体的宫颈光滑妇女40例为对照组。所有受检者均行阴道镜下宫颈活检,作出组织病理学诊断,采用荧光原位杂交技术检测宫颈组织中hTERC基因表达情况。结果随着宫颈糜烂程度的加重,宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)Ⅲ及宫颈癌的阳性率呈逐渐升高的趋势,各组间不同病理分级的阳性率差异均有统计学意义(P均<0.05);宫颈糜烂组与对照组宫颈活检组织标本均可见hTERC基因表达的细胞;在100例受检者中,56例hTERC基因扩增阳性,阳性表达率为56.00%(56/100)。在对照组、轻度糜烂组、中度糜烂组及重度糜烂组中hTERC基因的阳性表达率分别为10.00%(4/40)、68.42%(13/19)、91.30%(21/23)及100.00%(18/18),四组间比较差异具有统计学意义(χ2=41.283,P<0.05);且宫颈活检组织中hTERC基因与宫颈糜烂有较好的相关性,相关系数r为0.936(P=0.000)。宫颈活检组织中hTERC基因对宫颈糜烂的诊断性能较好,灵敏度及特异性分别为87.03%和83.33%。结论宫颈糜烂的患者其组织学诊断多样化,糜烂程度越高,发生CIN及宫颈癌的可能性越大,hTERC基因表达水平亦有随之升高的趋势。Objective To explore the clinical significance and expression of the human telomerase RNA component（hTERC） gene in postmenopausal women with cervical erosion.Methods 60 cases postmenopausal women with cervical erosion by naked eye view from February 2015 to August 2015 in our hospital were collected,and they were divided into mild cervical erosion group（19 cases）,moderate erosion group（23cases）,severe erosion group（18 cases）.40 cases patients with no cervical erosion were collected as the control group at the same time.All the subjects were all detected by colposcopy and cervical biopsy.The h TERC gene expression was detected by fluorescence in situ hybridization（FISH） technique.Results All the patients were divided into normal,cervicitis,cervical intraepithelial neoplasia（CIN） Ⅰ,CINⅡ,CINⅢ and cervical cancer according to the results of cervical biopsy pathology.The positive rates of CIN Ⅲ and cervical cancer showed a increasing trend along with the severity of disease.There were statistical significance in the differences of positive rate of different pathology degree among all the groups（Pall 〈0.05）.The samples of each cervical erosion group and control group all showed h TERC gene expression.There were 56 cases patients showed h TERC gene expression,the positive rate was 56.00%（56 / 100）.The positive rates of control group,mild cervical erosion group,moderate erosion group and severe erosion group were 10.00%（4 / 40）,68.42%（13 / 19）,91.30%（21 / 23） and 100.00%（18 / 18）,and the difference had statistical significance（χ2= 41.283,P〈 0.05）.There was positive correlation between cervical erosion and h TERC gene expression（r= 0.936,P= 0.000）.The sensitivity and specificity of hTERC gene in cervical erosion diagnosis were 87.03% and 83.33%,respectively.Conclusion Cervical erosion patients with the naked eye diagnosed show diverse histological diagnosis,the higher the degree of erosion,the greater the likelihood of CIN and cervical cancer,and hTERC g

关 键 词：绝经后 妇女 宫颈糜烂 宫颈上皮内瘤样病变 人类染色体末端酶基因 

分 类 号：R711.32[医药卫生—妇产科学]