冬虫夏草繁育品与野生品红外指纹图谱一致性评价  

Consistency Evaluation of IR Fingerprints of Cultivated Cordyceps and Natural Cordyceps

作　　者：詹泽苹 李华 黄亮 李文庆 辛立波 李文佳 钱正明 刘国柱 邓炳初 

机构地区：[1]广东东阳光药业有限公司,广东东莞523808 [2]广东省食品药品检验所,广东广州510180

出　　处：《中国现代中药》2016年第3期312-315,共4页Modern Chinese Medicine

基　　金：中医药行业科研专项(201507002);创新药物研发与产业化团队(201001Y0104742262)

摘　　要：目的：建立冬虫夏草的红外指纹图谱检测方法,对冬虫夏草野生品、繁育品以及伪虫草（蝉花、虫草花、中华被毛孢菌丝粉、蝙蝠蛾拟青霉菌丝粉）进行红外测试。方法：根据红外平均图谱、一阶导数图谱、二阶导数图谱和标准偏差图谱,判断这3种虫草的一致性。结果：在3000~2800 cm-1、1800~1000 cm-1、1000~800 cm-1,30批冬虫夏草繁育品和21批野生品的红外光谱图谱的指纹图谱具有较好的一致性,相似度均大于0.90;与伪虫草不一致,相似度均小于0.90。结论：该方法具有良好的专属性、重复性、稳定性和精密度,可用于冬虫夏草红外指纹图谱分析。Objective： In this research, an IR fingerprint analysis method was developed for identifying Cordyceps. Methods： Natural Cordyceps, cultivated Cordyceps and pseudo Cordyceps（ cicada fungus, Cordyceps flower,hirsutella mycelium powder and paecilomyces hepiali mycelium powder） were identified respectively by this method. The conformity of the samples were estimated according to the similarity of IR average spectra,first-order derivative spectra and second derivative spectra. Results： The IR fingerprint had good uniformity between 30 batches of cultivated Cordyceps and natural samples in the range of 3000-2800 cm- 1,1800-1000 cm- 1and 1000-800 cm- 1,with similarity not less than0. 90. However,the pseudo Cordyceps obtained inconsistent results,with similarity less than 0. 90. Conclusion： The method is proved specific,sensitive and accurate,which is suitable for identification of Cordyceps.

关 键 词：冬虫夏草 红外指纹图谱 一致性分析 

分 类 号：R284.1[医药卫生—中药学]