不同类型鼻息肉患者外周血Th17细胞含量的变化及意义  

Change of peripheral blood Th17 cells and its significance in patients with different kinds of chronic rhinosinusitis with nasal polyps

作　　者：邹华[1] 刘翔[1] 田鹏[1] 陈美恒[1] 潘忠[1] 谢存存[1] 林泓霖[1] 

机构地区：[1]中山大学孙逸仙纪念医院耳鼻咽喉头颈外科,广东广州510120

出　　处：《中国耳鼻咽喉头颈外科》2016年第4期230-234,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：广东省自然科学基金自由申请项目(2014A030313158)

摘　　要：目的研究不同类型鼻息肉患者外周血Th17细胞含量及其与炎性细胞浸润的关系;探讨其与预后的关系。方法对81例鼻息肉和20例鼻中隔偏曲患者进行研究。外周血Th17细胞含量采用流式细胞仪检测;鼻息肉组织嗜酸性粒细胞、中性粒细胞、淋巴细胞、浆细胞浸润程度以每高倍视野每10~4μm^2面积所含有的数量表示;内镜表现采用Lund-Kennedy评分;主观症状采用VAS评分;影像学CT表现采用Lund-Mackay评分。结果嗜酸性鼻息肉组和非嗜酸性鼻息肉组患者外周血Th17细胞百分比分别为2.10%(3.75%,1.40%)和1.10%(1.70%,0.73%),两组差异有统计学意义(Mann-Whitney U=358.0,Z=-2.965,P=0.001);外周血Th17细胞百分比与鼻息肉组织中嗜酸性粒细胞呈正相关(r=0.408,P<0.001),与术后3个月内镜评分有中等程度相关性(r=0.458,P<0.001)。结论 Thl7细胞表达可能与噬酸性鼻息肉发病及预后有关。OBJECTIVE To observe the change of peripheral blood Th17 cells in patients with different kinds of CRSw NPs and the relationship between the frequency of Th17 cells and inflammatory cell density in nasal polyps tissue,and to explore the cor relation between levels of per ipheral blood T h17 cells and prog nosis of patients with CRSw NPs.METHODS Eighty one patients with CRSw NPs and 20 controls were recruited in this research.Flow cy tometer was used to detect t he expression of peripheral blood Th17 cells.The number per 10000μm^2 of infiltrated inflammatory cells in nasal polyp tissue（including eosinophils,neutrophils,lymphocytes and plasma cells） was counted at a high-power field.The CT scores were evaluated by Lund-Mackay system and the nasal endoscopy scores were graded according to Lund-Mackay methods.RESULTS The percentages of Th17 cells in patients with E-CRSw NPs and NE-CRSw NPs were 2.10%（3.75%,1.40%）和1.10%（1.70%,0.73%）.There was significant difference between the two groups（Mann-Whitney U =358.0,Z =-2.965,P = 0.001）.Fur ther more,a positive association between the percentage of Th17 cells in peripheral blood and the eosinophil density of nasal polyp（r =0.408,P〈0.001） was demonstrated.The percentage of Th17 cell in peripheral blood was significantly correlated with the endoscopic score of CRSw NPs at third month after the operation（r =0.458,P〈0.001）.CONCLUSION Thl7 might be involved in the pathogenesis and prognosisof eosinophilic CRSw NPs.

关 键 词：鼻息肉 鼻窦炎 T淋巴细胞 辅助诱导 流式细胞术 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]