埃及血吸虫基因组内存在着曼氏血吸虫基因组“特异的”DNA重复序列  

THE REPETITIVE DNA SEQUENCES "SPECIFIC" TO THE GENOME OF SCHISTOSOMA MANSONI ARE ALSO PRESENTED IN THE GENOME OF SCHISTOSOMA HAEMATOBIUM

作　　者：何纳[1] 姜庆五[1] 赵根明[1] 刘建翔[1] 韦建国[1] 赵春琳[1] Joseph Hamburger 

机构地区：[1]复旦大学公共卫生学院流行病学教研室,上海200032 [2]以色列希伯来大学Kuvin传染病与热带病研究中心

出　　处：《中国血吸虫病防治杂志》2002年第4期252-255,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金 ( 39870 6 5 5 )~~

摘　　要：目的　探索埃及血吸虫基因组内是否存在曼氏血吸虫基因组“特异的”高度重复序列Sm1- 7。方法　以埃及血吸虫基因组 DNA为模板 ,用根据 Sm1- 7设计并合成的寡核苷酸引物进行多聚酶链式反应 (PCR)扩增 ,选取扩增产物中长约 30 0 bp的 DNA片段 ,经过预处理后与去磷酸化的质粒载体 p Bluescript (KS+)进行连接 ,连接产物转化至大肠杆菌 JM10 9。重组质粒经纯化后作DNA序列测定。结果　从埃及血吸虫基因组 DNA中成功地扩增出与曼氏血吸虫基因组重复序列Sm1- 7同源的重复序列。通过 DNA序列测定显示 ,除个别碱基不同外 ,从埃及血吸虫基因组获得的重复序列与曼氏血吸虫基因组重复序列 Sm1- 7完全相同。灵敏度分析显示该重复序列在埃及血吸虫基因组内的拷贝数远低于其在曼氏血吸虫基因组内的拷贝数。结论　曼氏血吸虫基因组“特异的”DNA重复序列 Sm1-Objective To determine whether the repetitive DNA sequences 'specific' to the genome of Schistosoma mansoni are also presented in the genome of Schistosoma haematobium.Methods Polymerase Chain Reaction (PCR) assay was done to amplify the S. mansoni-specific repetitive DNA sequences from the genome of S. haematobium.The oligo-nucleotide primers were designed based on the S. mansoni-specific repeated DNA fragment (Sm1-7).The 300 bp PCR product was extracted,purified and treated in order to be ligated with dephosphorylated plasmid vector (pBluescript Ⅱ). The recombinant plasmid was then transformed into the host bacteria JM109. The purified recombinant plasmid DNA was then subjected to DNA sequence analysis. Results S. mansoni -specific repeated DNA sequences(Sm1-7) were successfully amplified from the genome of S. haematobium. Only several point mutations were observed in the amplified and sequenced repetitive DNA from the genome of S. haematobium, compared with its origins (Sm1-7). Sensitivity analysis shows that the copies of this repeated DNA sequence fragment are far less in the genome of S.haematobium than that in the genome of S.mansoni. Conclusion The repetitive DNA sequences speciffic to the genome of S.mansoni are also presented in the genome of S.haematobium.

关 键 词：DNA 重复序列 血吸虫基因组 多聚酶链式反应 

分 类 号：R383.24[医药卫生—医学寄生虫学]