高脂血症大鼠病程进展的尿液代谢组学研究  

Study of the urine metabonomics on the progression of disease in hyperlipidemia rats

作　　者：罗朵生[1,2] 李坤平[2] 朴胜华[1,2] 郭姣[1,2] 

机构地区：[1]广州中医药大学/广东省代谢性疾病中医药防治重点实验室,广东广州510006 [2]广东药科大学中医药研究院/广东省代谢病中西医结合研究中心,广东广州510006

出　　处：《广东药学院学报》2016年第2期223-227,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金青年项目(81503313;81202619);广州市科技计划项目(1563000412);广东药学院创新强校资助项目

摘　　要：目的采用超高效液相色谱-飞行时间质谱代谢组学法动态观察高脂血症大鼠造模过程中尿液的代谢轮廓的改变,寻找高脂血症演进过程中的生物标志物。方法将SD大鼠分成正常对照组和模型组,运用高脂乳剂灌胃法复制高脂血症大鼠模型,并收集不同时间点的尿样,采用超高效液相色谱-飞行时间质谱仪检测并进行多元数据分析。结果随着造模时间的延长,高脂血症大鼠血浆代谢轮廓呈现动态演变,与正常组相比,模型组3-羟基丁酸、乙酸乙酰、丙酮酸、肌酐、N-氧化烟酰胺明显升高,而柠檬酸、马尿酸、牛磺酸以及3-吲哚硫酸、肌酸、乌头酸明显下降。结论在高脂血症大鼠病程进展过程中,出现了能量代谢紊乱、炎症和氧化应激。该研究加深了对高脂血症发病机制的认识,为研发有效防控高脂血症策略提供科学依据。Objective To observe the progression of hyperlipidemia and identify potential biomarkers in urine,UPLC / Q-TOF / MS metabonomics was used to study the metabolic changes. Methods Hyperlipidemia rats were established by intragastric administration of fat emulsion. Urine samples were collected at different times during the development of hyperlipidemia and detected by UPLC / Q-TOF / MS. The data was evaluated by multivariate analyses. Results The metabolic changes were gradually evident with time prolonging in hyperlipidemia rats. Compared with normal rats,the levels of 3-hydroxybutyric acid,acetic acid acetyl,pyroracemic acid,creatinine and N-oxidation of nicotinamide were increased,but the levels of citric acid,benzoyl-glycine,ethylamine sulfonic acid,3-indole sulfuric acid,creatine and aconitic acid were decreased in hyperlipidemia rats. Conclusion The metabolic disorders,inflammation and oxidative stress are gradually apparent on the development of hyperlipidemia. These findings provide an evidence for understanding the mechanism of hyperlipidemia and its countermeasures.

关 键 词：高脂血症 代谢组学 超高效液相色谱-飞行时间质谱 

分 类 号：R589.2[医药卫生—内分泌]