基于过敏性鼻炎大鼠模型优选鼻鼽颗粒提取工艺  

Optimization on Extraction Technology of Biqiu Granules based on Rat Model of Allergic Rhinitis

作　　者：刘莉莉[1,2,3] 张艳军[1,2,3] 常秀娟[1,2,3] 李雪峰[1,2,3] 李月婧 刘芳 王振中[1,2,3] 

机构地区：[1]南京中医药大学药学院,南京210000 [2]中药制药过程新技术国家重点实验室,连云港222001 [3]中药提取精制新技术重点研究室,连云港222001 [4]江苏康缘阳光药业有限公司,南京210000

出　　处：《世界科学技术-中医药现代化》2016年第3期505-510,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部"重大新药创制"科技重大专项(2013ZX09402203):现代中药创新集群与数字制药技术平台;负责人:王振中

摘　　要：目的:应用效应面法及药效筛选法优选鼻鼽颗粒的提取工艺。方法:采用效应面法的方法,以迷迭香酸提取率为指标,通过单因素试验和Box-Behnken效应面法考察提取溶剂量、提取时间、提取次数对提取工艺的影响,进一步通过药效筛选确定鼻鼽颗粒的疗效。结果:与模型组比较,鼻鼽颗粒工艺血清细胞因子水平均有下降趋势,可降低Ig E和IFN差异有统计学意义(P<0.05,P<0.01)。最佳提取工艺为提取溶剂量2 430 m L(9倍量),提取2次,每次1.5 h。迷迭香酸提取率84.89%。结论 :鼻鼽颗粒在降低免疫因子水平方面疗效明确,通过综合评价提取物的治疗效果,优选的鼻鼽颗粒工艺提取工艺稳定、可行。This study was aimed to optimize the extraction process of the Biqiu granules by response surface methodology（RSM） and activity screening method. With the rosemary acid extraction rate as index, the solvent volume, extraction duration and extraction times were investigated with the single factor assays and Box-Behnken method. The activity screening method was further utilized to determine the curative effect of Biqiu granules. The results showed that compared with the model group, the Biqiu granules had the descending trend on serum cytokines（Ig E and IFN） with statistical significance（P〈 0.05, P〈 0.01）. The optimal extraction process of Biqiu granules was shown as follows： solvent volume 2430 ml（9-fold）, extraction duration for 1.5 h, twice. The rosemary acid extraction rate was 84.89%. It was concluded that Biqiu granules had specific curative efficacy on reducing the levels of immune factors and its optimal extraction process was stable and feasible.

关 键 词：效应面法 迷迭香酸 PLACKETT-BURMAN Box-Behnken 药效筛选 

分 类 号：R285.5[医药卫生—中药学]