参芎通脉颗粒对脑缺血再灌注大鼠细胞凋亡指标P53、Fas蛋白表达的影响  

Effect of Shenxiong Tongmai granules on expression of apoptosis indices p53 and Fas in rats with cerebral ischemia / reperfusion

作　　者：王佳恒[1] 陈卫银[2] 

机构地区：[1]成都中医药大学,四川成都610075 [2]成都中医药大学附属医院,四川成都610072

出　　处：《湖南中医杂志》2016年第4期161-163,共3页Hunan Journal of Traditional Chinese Medicine

摘　　要：目的:探讨参芎通脉颗粒对局灶性脑缺血再灌注模型大鼠细胞凋亡的抑制作用。方法:将60只雄性SD大鼠随机分为空白组、假手术组、模型Ⅰ组(0.9%氯化钠注射液腹腔注射7d后行造模再灌注)、模型Ⅱ组(造模再灌注后以0.9%氯化钠注射液腹腔注射3d)、预处理组(50%参芎通脉颗粒溶液灌胃7d后行造模再灌注)、治疗组(造模再灌注后以50%参芎通脉颗粒溶液灌胃3d);用免疫组化法测定各组肿瘤抑制基因p53、脂肪酸合成酶(Fas)的表达水平。结果:参芎通脉颗粒预处理及治疗均能减轻局灶性脑缺血模型的神经损伤,与模型组相比较,预处理组及治疗组p53、Fas的表达降低(P<0.05)。结论:参芎通脉颗粒预处理和治疗均能抑制局灶性脑缺血模型SD大鼠神经细胞的凋亡,具有神经保护作用。Objective： To investigate the inhibitory effect of Shenxiong Tongmai granules on apoptosis in the rat model of focal cerebral ischemia- reperfusion. Methods： A total of 60 male Sprague- Dawley rats were randomly divided into blank group,sham- operation group,model I group（ reperfusion was performed after 7 days of intraperitoneal injection of 0. 9% sodium chloride injection）,model II group（ intraperitoneal injection of 0. 9% sodium chloride injection was performed for 3 days after reperfusion）,pretreatment group（ reperfusion was performed after 7 days of gavage of 50% Shenxiong Tongmai granule solution）,and treatment group（ 50% Shenxiong Tongmai granule solution was given by gavage for 3 days after reperfusion）. Immunohistochemistry was used to measure the expression of tumor suppressor gene p53 and fatty acid synthase（ Fas） in each group. Results： Pretreatment and treatment with Shenxiong Tongmai granules reduced nerve injury in the rat model of focal cerebral ischemia,and compared with the model group,the pretreatment group and treatment group showed significant reductions in the expression of p53 and Fas（ P〈0. 05）. Conclusion： Pretreatment and treatment with Shenxiong Tongmai granules can inhibit apoptosis of neural cells in Sprague- Dawley rats with focal cerebral ischemia and have a neuroprotective effect.

关 键 词：脑缺血再灌注 大鼠 参芎通脉颗粒 细胞凋亡 P53 Fas 

分 类 号：R285.5[医药卫生—中药学]